Multiplex allele-specific polymerase chain reaction for detection of isoniazid resistance in Mycobacterium tuberculosis.
SETTING: Pham Ngoc Thach Tuberculosis Reference Hospital, Ho Chi Minh City, Viet Nam. DESIGN: A multiplex allele-specific polymerase chain reaction (MAS-PCR) was developed to detect mutations at the two most common sites responsible for isoniazid (INH) resistance in Mycobacterium tuberculosis: katG...
| Hauptverfasser: | , , , , |
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| Format: | Journal article |
| Sprache: | English |
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2011
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| _version_ | 1826301683392577536 |
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| author | Tho, D Lan, N Chau, N Farrar, J Caws, M |
| author_facet | Tho, D Lan, N Chau, N Farrar, J Caws, M |
| author_sort | Tho, D |
| collection | OXFORD |
| description | SETTING: Pham Ngoc Thach Tuberculosis Reference Hospital, Ho Chi Minh City, Viet Nam. DESIGN: A multiplex allele-specific polymerase chain reaction (MAS-PCR) was developed to detect mutations at the two most common sites responsible for isoniazid (INH) resistance in Mycobacterium tuberculosis: katG315 and inhA-15. The MAS-PCR is able to detect rare mutations at katG315, in addition to katG S315T. Conventional phenotypic proportion drug susceptibility testing on Löwenstein-Jensen media was used as a gold standard to compare the sensitivity and specificity of the commercial MTBDRplus line-probe assay and the MAS-PCR in 100 INH-resistant and 50 INH-susceptible isolates collected consecutively at Pham Ngoc Thach Hospital reference laboratory. RESULTS: The sensitivity and specificity on culture isolates were 90% (n = 90/100, 95%CI 0.83-0.94) and 100% (n = 50/50, 95%CI 0.93-1.0), respectively, for the MAS-PCR and the MTBDRplus assay. CONCLUSION: The MAS-PCR described here represents an alternative method for rapid screening for INH resistance in M. tuberculosis isolates. |
| first_indexed | 2024-03-07T05:36:04Z |
| format | Journal article |
| id | oxford-uuid:e3eeb45a-981e-403d-a00a-d9f6f8663746 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:36:04Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:e3eeb45a-981e-403d-a00a-d9f6f86637462022-03-27T10:12:50ZMultiplex allele-specific polymerase chain reaction for detection of isoniazid resistance in Mycobacterium tuberculosis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e3eeb45a-981e-403d-a00a-d9f6f8663746EnglishSymplectic Elements at Oxford2011Tho, DLan, NChau, NFarrar, JCaws, M SETTING: Pham Ngoc Thach Tuberculosis Reference Hospital, Ho Chi Minh City, Viet Nam. DESIGN: A multiplex allele-specific polymerase chain reaction (MAS-PCR) was developed to detect mutations at the two most common sites responsible for isoniazid (INH) resistance in Mycobacterium tuberculosis: katG315 and inhA-15. The MAS-PCR is able to detect rare mutations at katG315, in addition to katG S315T. Conventional phenotypic proportion drug susceptibility testing on Löwenstein-Jensen media was used as a gold standard to compare the sensitivity and specificity of the commercial MTBDRplus line-probe assay and the MAS-PCR in 100 INH-resistant and 50 INH-susceptible isolates collected consecutively at Pham Ngoc Thach Hospital reference laboratory. RESULTS: The sensitivity and specificity on culture isolates were 90% (n = 90/100, 95%CI 0.83-0.94) and 100% (n = 50/50, 95%CI 0.93-1.0), respectively, for the MAS-PCR and the MTBDRplus assay. CONCLUSION: The MAS-PCR described here represents an alternative method for rapid screening for INH resistance in M. tuberculosis isolates. |
| spellingShingle | Tho, D Lan, N Chau, N Farrar, J Caws, M Multiplex allele-specific polymerase chain reaction for detection of isoniazid resistance in Mycobacterium tuberculosis. |
| title | Multiplex allele-specific polymerase chain reaction for detection of isoniazid resistance in Mycobacterium tuberculosis. |
| title_full | Multiplex allele-specific polymerase chain reaction for detection of isoniazid resistance in Mycobacterium tuberculosis. |
| title_fullStr | Multiplex allele-specific polymerase chain reaction for detection of isoniazid resistance in Mycobacterium tuberculosis. |
| title_full_unstemmed | Multiplex allele-specific polymerase chain reaction for detection of isoniazid resistance in Mycobacterium tuberculosis. |
| title_short | Multiplex allele-specific polymerase chain reaction for detection of isoniazid resistance in Mycobacterium tuberculosis. |
| title_sort | multiplex allele specific polymerase chain reaction for detection of isoniazid resistance in mycobacterium tuberculosis |
| work_keys_str_mv | AT thod multiplexallelespecificpolymerasechainreactionfordetectionofisoniazidresistanceinmycobacteriumtuberculosis AT lann multiplexallelespecificpolymerasechainreactionfordetectionofisoniazidresistanceinmycobacteriumtuberculosis AT chaun multiplexallelespecificpolymerasechainreactionfordetectionofisoniazidresistanceinmycobacteriumtuberculosis AT farrarj multiplexallelespecificpolymerasechainreactionfordetectionofisoniazidresistanceinmycobacteriumtuberculosis AT cawsm multiplexallelespecificpolymerasechainreactionfordetectionofisoniazidresistanceinmycobacteriumtuberculosis |