Novel method for detection, typing, and quantification of human papillomaviruses in clinical samples.
We report the development of a novel detection and typing methodology for human papillomaviruses (HPV) based on real-time PCR with the self-probing fluorescent primers known as Scorpions. This technique is quick, simple, specific, sensitive, and capable of estimating viral load per cell. We report t...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2001
|
| _version_ | 1826301770970693632 |
|---|---|
| author | Hart, K Williams, O Thelwell, N Fiander, A Brown, T Borysiewicz, L Gelder, C |
| author_facet | Hart, K Williams, O Thelwell, N Fiander, A Brown, T Borysiewicz, L Gelder, C |
| author_sort | Hart, K |
| collection | OXFORD |
| description | We report the development of a novel detection and typing methodology for human papillomaviruses (HPV) based on real-time PCR with the self-probing fluorescent primers known as Scorpions. This technique is quick, simple, specific, sensitive, and capable of estimating viral load per cell. We report the results of over 100 typing reactions performed on cell lines, biopsies, and cervical cytobrush samples which, when compared to the current reference HPV detection and typing technique, present a kappa value of 0.89. We further report preliminary data suggesting a relationship between viral load per cell and grade of cervical disease. |
| first_indexed | 2024-03-07T05:37:24Z |
| format | Journal article |
| id | oxford-uuid:e4620b2b-f04c-470c-8908-ee0d69e16f3a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:37:24Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | oxford-uuid:e4620b2b-f04c-470c-8908-ee0d69e16f3a2022-03-27T10:16:11ZNovel method for detection, typing, and quantification of human papillomaviruses in clinical samples.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e4620b2b-f04c-470c-8908-ee0d69e16f3aEnglishSymplectic Elements at Oxford2001Hart, KWilliams, OThelwell, NFiander, ABrown, TBorysiewicz, LGelder, CWe report the development of a novel detection and typing methodology for human papillomaviruses (HPV) based on real-time PCR with the self-probing fluorescent primers known as Scorpions. This technique is quick, simple, specific, sensitive, and capable of estimating viral load per cell. We report the results of over 100 typing reactions performed on cell lines, biopsies, and cervical cytobrush samples which, when compared to the current reference HPV detection and typing technique, present a kappa value of 0.89. We further report preliminary data suggesting a relationship between viral load per cell and grade of cervical disease. |
| spellingShingle | Hart, K Williams, O Thelwell, N Fiander, A Brown, T Borysiewicz, L Gelder, C Novel method for detection, typing, and quantification of human papillomaviruses in clinical samples. |
| title | Novel method for detection, typing, and quantification of human papillomaviruses in clinical samples. |
| title_full | Novel method for detection, typing, and quantification of human papillomaviruses in clinical samples. |
| title_fullStr | Novel method for detection, typing, and quantification of human papillomaviruses in clinical samples. |
| title_full_unstemmed | Novel method for detection, typing, and quantification of human papillomaviruses in clinical samples. |
| title_short | Novel method for detection, typing, and quantification of human papillomaviruses in clinical samples. |
| title_sort | novel method for detection typing and quantification of human papillomaviruses in clinical samples |
| work_keys_str_mv | AT hartk novelmethodfordetectiontypingandquantificationofhumanpapillomavirusesinclinicalsamples AT williamso novelmethodfordetectiontypingandquantificationofhumanpapillomavirusesinclinicalsamples AT thelwelln novelmethodfordetectiontypingandquantificationofhumanpapillomavirusesinclinicalsamples AT fiandera novelmethodfordetectiontypingandquantificationofhumanpapillomavirusesinclinicalsamples AT brownt novelmethodfordetectiontypingandquantificationofhumanpapillomavirusesinclinicalsamples AT borysiewiczl novelmethodfordetectiontypingandquantificationofhumanpapillomavirusesinclinicalsamples AT gelderc novelmethodfordetectiontypingandquantificationofhumanpapillomavirusesinclinicalsamples |