Potentially important microRNA cluster on chromosome 17p13.1 in primary peritoneal carcinoma.
MicroRNAs are a group of small non-coding RNAs approximately 22 nucleotides in length. Recent work has shown differential expression of mature microRNAs in human cancers. We characterized the alteration in expression of a select group of microRNAs in primary peritoneal carcinoma relative to matched...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2009
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| _version_ | 1797100446285824000 |
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| author | Flavin, R Smyth, P Laios, A O'Toole, SA Barrett, C Finn, S Russell, S Ring, M Denning, K Li, J Aherne, S Sammarae, D Aziz, N Alhadi, A Sheppard, B Lao, K Sheils, O O'Leary, J |
| author_facet | Flavin, R Smyth, P Laios, A O'Toole, SA Barrett, C Finn, S Russell, S Ring, M Denning, K Li, J Aherne, S Sammarae, D Aziz, N Alhadi, A Sheppard, B Lao, K Sheils, O O'Leary, J |
| author_sort | Flavin, R |
| collection | OXFORD |
| description | MicroRNAs are a group of small non-coding RNAs approximately 22 nucleotides in length. Recent work has shown differential expression of mature microRNAs in human cancers. We characterized the alteration in expression of a select group of microRNAs in primary peritoneal carcinoma relative to matched cases of ovarian serous carcinoma. MicroRNA expression was analysed using semi-quantitative stem-loop RT-PCR on a set of 34 formalin-fixed paraffin-embedded samples. Protein expression of p53 and bcl-2 was quantified in the corresponding tissue microarray. We provide definitive evidence that there is downregulation of a select group of microRNAs in tumours meeting Gynaecological Oncology Group criteria for primary peritoneal carcinoma relative to ovarian serous carcinoma. Specifically, we show decreased p53 expression and downregulation of miR-195 and miR-497 from the microRNA cluster site at chromosome 17p13.1 in primary peritoneal carcinoma relative to ovarian serous carcinoma. miR-195 and miR-497 may have potential roles as tumour-suppressor genes in primary peritoneal tumourigenesis. |
| first_indexed | 2024-03-07T05:37:37Z |
| format | Journal article |
| id | oxford-uuid:e475630a-f2b6-4bd4-9fb5-f03ef1568b45 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:37:37Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:e475630a-f2b6-4bd4-9fb5-f03ef1568b452022-03-27T10:16:48ZPotentially important microRNA cluster on chromosome 17p13.1 in primary peritoneal carcinoma.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e475630a-f2b6-4bd4-9fb5-f03ef1568b45EnglishSymplectic Elements at Oxford2009Flavin, RSmyth, PLaios, AO'Toole, SABarrett, CFinn, SRussell, SRing, MDenning, KLi, JAherne, SSammarae, DAziz, NAlhadi, ASheppard, BLao, KSheils, OO'Leary, JMicroRNAs are a group of small non-coding RNAs approximately 22 nucleotides in length. Recent work has shown differential expression of mature microRNAs in human cancers. We characterized the alteration in expression of a select group of microRNAs in primary peritoneal carcinoma relative to matched cases of ovarian serous carcinoma. MicroRNA expression was analysed using semi-quantitative stem-loop RT-PCR on a set of 34 formalin-fixed paraffin-embedded samples. Protein expression of p53 and bcl-2 was quantified in the corresponding tissue microarray. We provide definitive evidence that there is downregulation of a select group of microRNAs in tumours meeting Gynaecological Oncology Group criteria for primary peritoneal carcinoma relative to ovarian serous carcinoma. Specifically, we show decreased p53 expression and downregulation of miR-195 and miR-497 from the microRNA cluster site at chromosome 17p13.1 in primary peritoneal carcinoma relative to ovarian serous carcinoma. miR-195 and miR-497 may have potential roles as tumour-suppressor genes in primary peritoneal tumourigenesis. |
| spellingShingle | Flavin, R Smyth, P Laios, A O'Toole, SA Barrett, C Finn, S Russell, S Ring, M Denning, K Li, J Aherne, S Sammarae, D Aziz, N Alhadi, A Sheppard, B Lao, K Sheils, O O'Leary, J Potentially important microRNA cluster on chromosome 17p13.1 in primary peritoneal carcinoma. |
| title | Potentially important microRNA cluster on chromosome 17p13.1 in primary peritoneal carcinoma. |
| title_full | Potentially important microRNA cluster on chromosome 17p13.1 in primary peritoneal carcinoma. |
| title_fullStr | Potentially important microRNA cluster on chromosome 17p13.1 in primary peritoneal carcinoma. |
| title_full_unstemmed | Potentially important microRNA cluster on chromosome 17p13.1 in primary peritoneal carcinoma. |
| title_short | Potentially important microRNA cluster on chromosome 17p13.1 in primary peritoneal carcinoma. |
| title_sort | potentially important microrna cluster on chromosome 17p13 1 in primary peritoneal carcinoma |
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