Finding and verifying enhancers for endothelial-expressed genes

Identification and analysis of enhancers for endothelial-expressed genes can provide crucial information regarding their upstream transcriptional regulators. However, enhancer identification can be challenging, particularly for people with limited access or experience of bioinformatics, and transgen...

Full description

Bibliographic Details
Main Authors: Neal, A, Rodriguez-Caro, H, De Val, S
Other Authors: Benest, AV
Format: Book section
Language:English
Published: Humana Press 2022
_version_ 1797112413320904704
author Neal, A
Rodriguez-Caro, H
De Val, S
author2 Benest, AV
author_facet Benest, AV
Neal, A
Rodriguez-Caro, H
De Val, S
author_sort Neal, A
collection OXFORD
description Identification and analysis of enhancers for endothelial-expressed genes can provide crucial information regarding their upstream transcriptional regulators. However, enhancer identification can be challenging, particularly for people with limited access or experience of bioinformatics, and transgenic analysis of enhancer activity patterns can be prohibitively expensive. Here we describe how to use publicly available datasets displayed on the UCSC Genome Browser to identify putative endothelial enhancers for mammalian genes. Furthermore, we detail how to utilize mosaic Tol2-mediated transgenesis in zebrafish to verify whether a putative enhancer is capable of directing endothelial-specific patterns of gene expression.
first_indexed 2024-03-07T08:23:52Z
format Book section
id oxford-uuid:e4c9ef22-5e85-4206-ac5e-bcf1ad0a3567
institution University of Oxford
language English
last_indexed 2024-03-07T08:23:52Z
publishDate 2022
publisher Humana Press
record_format dspace
spelling oxford-uuid:e4c9ef22-5e85-4206-ac5e-bcf1ad0a35672024-02-07T11:22:42ZFinding and verifying enhancers for endothelial-expressed genes Book sectionhttp://purl.org/coar/resource_type/c_3248uuid:e4c9ef22-5e85-4206-ac5e-bcf1ad0a3567EnglishSymplectic ElementsHumana Press2022Neal, ARodriguez-Caro, HDe Val, SBenest, AVIdentification and analysis of enhancers for endothelial-expressed genes can provide crucial information regarding their upstream transcriptional regulators. However, enhancer identification can be challenging, particularly for people with limited access or experience of bioinformatics, and transgenic analysis of enhancer activity patterns can be prohibitively expensive. Here we describe how to use publicly available datasets displayed on the UCSC Genome Browser to identify putative endothelial enhancers for mammalian genes. Furthermore, we detail how to utilize mosaic Tol2-mediated transgenesis in zebrafish to verify whether a putative enhancer is capable of directing endothelial-specific patterns of gene expression.
spellingShingle Neal, A
Rodriguez-Caro, H
De Val, S
Finding and verifying enhancers for endothelial-expressed genes
title Finding and verifying enhancers for endothelial-expressed genes
title_full Finding and verifying enhancers for endothelial-expressed genes
title_fullStr Finding and verifying enhancers for endothelial-expressed genes
title_full_unstemmed Finding and verifying enhancers for endothelial-expressed genes
title_short Finding and verifying enhancers for endothelial-expressed genes
title_sort finding and verifying enhancers for endothelial expressed genes
work_keys_str_mv AT neala findingandverifyingenhancersforendothelialexpressedgenes
AT rodriguezcaroh findingandverifyingenhancersforendothelialexpressedgenes
AT devals findingandverifyingenhancersforendothelialexpressedgenes