A photoswitchable solvatochromic dye for probing membrane ordering by RESOLFT super-resolution microscopy
A switchable solvatochromic fluorescent dyad can be used to map ordering of lipids in vesicle membranes at a resolution better than the diffraction limit. Combining a Nile Red fluorophore with a photochromic spironaphthoxazine quencher allows the fluorescence to be controlled using visible light, vi...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Wiley
2023
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| _version_ | 1826310453563752448 |
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| author | Frawley, AT Leslie, KG Wycisk, V Galiani, S Shrestha, D Eggeling, C Anderson, HL |
| author_facet | Frawley, AT Leslie, KG Wycisk, V Galiani, S Shrestha, D Eggeling, C Anderson, HL |
| author_sort | Frawley, AT |
| collection | OXFORD |
| description | A switchable solvatochromic fluorescent dyad can be used to map ordering of lipids in vesicle membranes at a resolution better than the diffraction limit. Combining a Nile Red fluorophore with a photochromic spironaphthoxazine quencher allows the fluorescence to be controlled using visible light, via photoswitching and FRET quenching. Synthetic lipid vesicles of varying composition were imaged with an average 2.5-fold resolution enhancement, compared to the confocal images. Ratiometric detection was used to probe the membrane polarity, and domains of different lipid ordering were distinguished within the same membrane. |
| first_indexed | 2024-03-07T07:51:21Z |
| format | Journal article |
| id | oxford-uuid:e4d841ba-6eaf-4c0e-ae6e-ec496d6532e7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:51:21Z |
| publishDate | 2023 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:e4d841ba-6eaf-4c0e-ae6e-ec496d6532e72023-07-17T10:17:55ZA photoswitchable solvatochromic dye for probing membrane ordering by RESOLFT super-resolution microscopyJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e4d841ba-6eaf-4c0e-ae6e-ec496d6532e7EnglishSymplectic ElementsWiley2023Frawley, ATLeslie, KGWycisk, VGaliani, SShrestha, DEggeling, CAnderson, HLA switchable solvatochromic fluorescent dyad can be used to map ordering of lipids in vesicle membranes at a resolution better than the diffraction limit. Combining a Nile Red fluorophore with a photochromic spironaphthoxazine quencher allows the fluorescence to be controlled using visible light, via photoswitching and FRET quenching. Synthetic lipid vesicles of varying composition were imaged with an average 2.5-fold resolution enhancement, compared to the confocal images. Ratiometric detection was used to probe the membrane polarity, and domains of different lipid ordering were distinguished within the same membrane. |
| spellingShingle | Frawley, AT Leslie, KG Wycisk, V Galiani, S Shrestha, D Eggeling, C Anderson, HL A photoswitchable solvatochromic dye for probing membrane ordering by RESOLFT super-resolution microscopy |
| title | A photoswitchable solvatochromic dye for probing membrane ordering by RESOLFT super-resolution microscopy |
| title_full | A photoswitchable solvatochromic dye for probing membrane ordering by RESOLFT super-resolution microscopy |
| title_fullStr | A photoswitchable solvatochromic dye for probing membrane ordering by RESOLFT super-resolution microscopy |
| title_full_unstemmed | A photoswitchable solvatochromic dye for probing membrane ordering by RESOLFT super-resolution microscopy |
| title_short | A photoswitchable solvatochromic dye for probing membrane ordering by RESOLFT super-resolution microscopy |
| title_sort | photoswitchable solvatochromic dye for probing membrane ordering by resolft super resolution microscopy |
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