Aquaporins in the wild: natural genetic diversity and selective pressure in the PIP gene family in five Neotropical tree species
<p style="text-align:justify;"> <b>Background:</b> Tropical trees undergo severe stress through seasonal drought and flooding, and the ability of these species to respond may be a major factor in their survival in tropical ecosystems, particularly in relation to global c...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
BioMed Central
2010
|
| _version_ | 1797100528247767040 |
|---|---|
| author | Audigeos, D Buonamici, A Belkadi, L Rymer, P Boshier, D Scotti-Saintagne, C Vendramin, G Scotti, I |
| author_facet | Audigeos, D Buonamici, A Belkadi, L Rymer, P Boshier, D Scotti-Saintagne, C Vendramin, G Scotti, I |
| author_sort | Audigeos, D |
| collection | OXFORD |
| description | <p style="text-align:justify;"> <b>Background:</b> Tropical trees undergo severe stress through seasonal drought and flooding, and the ability of these species to respond may be a major factor in their survival in tropical ecosystems, particularly in relation to global climate change. Aquaporins are involved in the regulation of water flow and have been shown to be involved in drought response; they may therefore play a major adaptive role in these species. We describe genetic diversity in the PIP sub-family of the widespread gene family of Aquaporins in five Neotropical tree species covering four botanical families.<br/><br/> <b>Results:</b> PIP Aquaporin subfamily genes were isolated, and their DNA sequence polymorphisms characterised in natural populations. Sequence data were analysed with statistical tests of standard neutral equilibrium and demographic scenarios simulated to compare with the observed results. Chloroplast SSRs were also used to test demographic transitions. Most gene fragments are highly polymorphic and display signatures of balancing selection or bottlenecks; chloroplast SSR markers have significant statistics that do not conform to expectations for population bottlenecks. Although not incompatible with a purely demographic scenario, the combination of all tests tends to favour a selective interpretation of extant gene diversity.<br/><br/> <b>Conclusions:</b> Tropical tree PIP genes may generally undergo balancing selection, which may maintain high levels of genetic diversity at these loci. Genetic variation at PIP genes may represent a response to variable environmental conditions. </p> |
| first_indexed | 2024-03-07T05:38:50Z |
| format | Journal article |
| id | oxford-uuid:e4df5e9b-d666-40bf-bf26-239a8429d9f9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:38:50Z |
| publishDate | 2010 |
| publisher | BioMed Central |
| record_format | dspace |
| spelling | oxford-uuid:e4df5e9b-d666-40bf-bf26-239a8429d9f92022-03-27T10:19:40ZAquaporins in the wild: natural genetic diversity and selective pressure in the PIP gene family in five Neotropical tree speciesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e4df5e9b-d666-40bf-bf26-239a8429d9f9EnglishSymplectic Elements at OxfordBioMed Central2010Audigeos, DBuonamici, ABelkadi, LRymer, PBoshier, DScotti-Saintagne, CVendramin, GScotti, I <p style="text-align:justify;"> <b>Background:</b> Tropical trees undergo severe stress through seasonal drought and flooding, and the ability of these species to respond may be a major factor in their survival in tropical ecosystems, particularly in relation to global climate change. Aquaporins are involved in the regulation of water flow and have been shown to be involved in drought response; they may therefore play a major adaptive role in these species. We describe genetic diversity in the PIP sub-family of the widespread gene family of Aquaporins in five Neotropical tree species covering four botanical families.<br/><br/> <b>Results:</b> PIP Aquaporin subfamily genes were isolated, and their DNA sequence polymorphisms characterised in natural populations. Sequence data were analysed with statistical tests of standard neutral equilibrium and demographic scenarios simulated to compare with the observed results. Chloroplast SSRs were also used to test demographic transitions. Most gene fragments are highly polymorphic and display signatures of balancing selection or bottlenecks; chloroplast SSR markers have significant statistics that do not conform to expectations for population bottlenecks. Although not incompatible with a purely demographic scenario, the combination of all tests tends to favour a selective interpretation of extant gene diversity.<br/><br/> <b>Conclusions:</b> Tropical tree PIP genes may generally undergo balancing selection, which may maintain high levels of genetic diversity at these loci. Genetic variation at PIP genes may represent a response to variable environmental conditions. </p> |
| spellingShingle | Audigeos, D Buonamici, A Belkadi, L Rymer, P Boshier, D Scotti-Saintagne, C Vendramin, G Scotti, I Aquaporins in the wild: natural genetic diversity and selective pressure in the PIP gene family in five Neotropical tree species |
| title | Aquaporins in the wild: natural genetic diversity and selective pressure in the PIP gene family in five Neotropical tree species |
| title_full | Aquaporins in the wild: natural genetic diversity and selective pressure in the PIP gene family in five Neotropical tree species |
| title_fullStr | Aquaporins in the wild: natural genetic diversity and selective pressure in the PIP gene family in five Neotropical tree species |
| title_full_unstemmed | Aquaporins in the wild: natural genetic diversity and selective pressure in the PIP gene family in five Neotropical tree species |
| title_short | Aquaporins in the wild: natural genetic diversity and selective pressure in the PIP gene family in five Neotropical tree species |
| title_sort | aquaporins in the wild natural genetic diversity and selective pressure in the pip gene family in five neotropical tree species |
| work_keys_str_mv | AT audigeosd aquaporinsinthewildnaturalgeneticdiversityandselectivepressureinthepipgenefamilyinfiveneotropicaltreespecies AT buonamicia aquaporinsinthewildnaturalgeneticdiversityandselectivepressureinthepipgenefamilyinfiveneotropicaltreespecies AT belkadil aquaporinsinthewildnaturalgeneticdiversityandselectivepressureinthepipgenefamilyinfiveneotropicaltreespecies AT rymerp aquaporinsinthewildnaturalgeneticdiversityandselectivepressureinthepipgenefamilyinfiveneotropicaltreespecies AT boshierd aquaporinsinthewildnaturalgeneticdiversityandselectivepressureinthepipgenefamilyinfiveneotropicaltreespecies AT scottisaintagnec aquaporinsinthewildnaturalgeneticdiversityandselectivepressureinthepipgenefamilyinfiveneotropicaltreespecies AT vendraming aquaporinsinthewildnaturalgeneticdiversityandselectivepressureinthepipgenefamilyinfiveneotropicaltreespecies AT scottii aquaporinsinthewildnaturalgeneticdiversityandselectivepressureinthepipgenefamilyinfiveneotropicaltreespecies |