Presentation of IgG2a antigens to class II-restricted T cells by stably transfected B lymphoma cells.
Here we describe a panel of BALB/c T cells specific for IgG2a of the b allotype in association with I-Ad. We used DNA-mediated gene transfer techniques to localize antigenic determinants recognized by responding T cells. Initially a truncated IgG2aa gene comprising a variable domain and the CH3 doma...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
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1989
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| _version_ | 1826301877146353664 |
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| author | Bikoff, E Eckhardt, L |
| author_facet | Bikoff, E Eckhardt, L |
| author_sort | Bikoff, E |
| collection | OXFORD |
| description | Here we describe a panel of BALB/c T cells specific for IgG2a of the b allotype in association with I-Ad. We used DNA-mediated gene transfer techniques to localize antigenic determinants recognized by responding T cells. Initially a truncated IgG2aa gene comprising a variable domain and the CH3 domain (not including the membrane exons) from the BALB/c IgG2aa heavy chain was introduced into myeloma cells. The V-CH3 protein was expressed at high levels under control of the Ig heavy chain enhancer. Secretion of the V-CH3 protein did not require assembly of H-H dimers or an association with light chains. To generate stably transfected B cell lines that would stimulate our class II-restricted T cells, we replaced most of the BALB/c IgG2aa CH3 exon with CH3 coding sequences from a C57BL/6 IgG2ab cDNA clone and introduced these constructs into Ia+ B lymphoma cells. The IgG2ab CH3-transfected B cells were recognized by BALB/c Igh-1b-specific T cell hybrids in the absence of exogenous antigen. Experiments using glutaraldehyde-fixed cells as stimulators indicate that presentation of the secreted form of V-IgG2ab CH3 requires processing. We found that a significant fraction of the endogenously synthesized V-IgG2ab CH3 protein was, however, present as already processed antigen. |
| first_indexed | 2024-03-07T05:39:02Z |
| format | Journal article |
| id | oxford-uuid:e4ed7710-92fa-44af-9308-3e0a9f2f496d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:39:02Z |
| publishDate | 1989 |
| record_format | dspace |
| spelling | oxford-uuid:e4ed7710-92fa-44af-9308-3e0a9f2f496d2022-03-27T10:20:04ZPresentation of IgG2a antigens to class II-restricted T cells by stably transfected B lymphoma cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e4ed7710-92fa-44af-9308-3e0a9f2f496dEnglishSymplectic Elements at Oxford1989Bikoff, EEckhardt, LHere we describe a panel of BALB/c T cells specific for IgG2a of the b allotype in association with I-Ad. We used DNA-mediated gene transfer techniques to localize antigenic determinants recognized by responding T cells. Initially a truncated IgG2aa gene comprising a variable domain and the CH3 domain (not including the membrane exons) from the BALB/c IgG2aa heavy chain was introduced into myeloma cells. The V-CH3 protein was expressed at high levels under control of the Ig heavy chain enhancer. Secretion of the V-CH3 protein did not require assembly of H-H dimers or an association with light chains. To generate stably transfected B cell lines that would stimulate our class II-restricted T cells, we replaced most of the BALB/c IgG2aa CH3 exon with CH3 coding sequences from a C57BL/6 IgG2ab cDNA clone and introduced these constructs into Ia+ B lymphoma cells. The IgG2ab CH3-transfected B cells were recognized by BALB/c Igh-1b-specific T cell hybrids in the absence of exogenous antigen. Experiments using glutaraldehyde-fixed cells as stimulators indicate that presentation of the secreted form of V-IgG2ab CH3 requires processing. We found that a significant fraction of the endogenously synthesized V-IgG2ab CH3 protein was, however, present as already processed antigen. |
| spellingShingle | Bikoff, E Eckhardt, L Presentation of IgG2a antigens to class II-restricted T cells by stably transfected B lymphoma cells. |
| title | Presentation of IgG2a antigens to class II-restricted T cells by stably transfected B lymphoma cells. |
| title_full | Presentation of IgG2a antigens to class II-restricted T cells by stably transfected B lymphoma cells. |
| title_fullStr | Presentation of IgG2a antigens to class II-restricted T cells by stably transfected B lymphoma cells. |
| title_full_unstemmed | Presentation of IgG2a antigens to class II-restricted T cells by stably transfected B lymphoma cells. |
| title_short | Presentation of IgG2a antigens to class II-restricted T cells by stably transfected B lymphoma cells. |
| title_sort | presentation of igg2a antigens to class ii restricted t cells by stably transfected b lymphoma cells |
| work_keys_str_mv | AT bikoffe presentationofigg2aantigenstoclassiirestrictedtcellsbystablytransfectedblymphomacells AT eckhardtl presentationofigg2aantigenstoclassiirestrictedtcellsbystablytransfectedblymphomacells |