Evaluation of the induction of immune memory following infant immunisation with serogroup C Neisseria meningitidis conjugate vaccines - exploratory analyses within a randomised controlled trial
AIM: We measured meningococcal serogroup C (MenC)-specific memory B-cell responses in infants by Enzyme-Linked Immunospot (ELISpot) following different MenC conjugate vaccine schedules to investigate the impact of priming on immune memory. METHODS: Infants aged 2 months were randomised to receive 1...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
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Public Library of Science
2014
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| _version_ | 1797100713046704128 |
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| author | Khatami, A Clutterbuck, E Thompson, A McKenna, J Pace, D Birks, J Snape, MD Pollard, A |
| author_facet | Khatami, A Clutterbuck, E Thompson, A McKenna, J Pace, D Birks, J Snape, MD Pollard, A |
| author_sort | Khatami, A |
| collection | OXFORD |
| description | AIM: We measured meningococcal serogroup C (MenC)-specific memory B-cell responses in infants by Enzyme-Linked Immunospot (ELISpot) following different MenC conjugate vaccine schedules to investigate the impact of priming on immune memory. METHODS: Infants aged 2 months were randomised to receive 1 or 2 doses of MenC-CRM197 at 3 or 3 and 4 months, 1 dose of MenC-TT at 3 months, or no primary MenC doses. All children received a Haemophilus influenzae type b (Hib)-MenC booster at 12 months. Blood was drawn at 5, 12, 12 months +6 days and 13 months of age. RESULTS: Results were available for 110, 103, 76 and 44 children from each group respectively. Following primary immunisations, and prior to the 12-month booster, there were no significant differences between 1- or 2-dose primed children in the number of MenC memory B-cells detected. One month following the booster, children primed with 1 dose MenC-TT had more memory B-cells than children primed with either 1-dose (p = 0.001) or 2-dose (p<0.0001) MenC-CRM197. There were no differences in MenC memory B-cells detected in children who received 1 or 2 doses of MenC-CRM197 in infancy and un-primed children. CONCLUSIONS: MenC-specific memory B-cell production may be more dependent on the type of primary vaccine used than the number of doses administered. Although the mechanistic differences between MenC-CRM197 and MenC-TT priming are unclear, it is possible that structural differences, including the carrier proteins, may underlie differential interactions with B- and T-cell populations, and thus different effects on various memory B-cell subsets. A MenC-TT/Hib-MenC-TT combination for priming/boosting may offer an advantage in inducing more persistent antibody. TRIAL REGISTRATION: EU Clinical Trials Register 2009-016579-31 ClinicalTrials.gov NCT01129518. |
| first_indexed | 2024-03-07T05:41:31Z |
| format | Journal article |
| id | oxford-uuid:e5b9ecff-fc13-43f9-baca-60396da9ac19 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:41:31Z |
| publishDate | 2014 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | oxford-uuid:e5b9ecff-fc13-43f9-baca-60396da9ac192022-03-27T10:26:09ZEvaluation of the induction of immune memory following infant immunisation with serogroup C Neisseria meningitidis conjugate vaccines - exploratory analyses within a randomised controlled trialJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e5b9ecff-fc13-43f9-baca-60396da9ac19EnglishSymplectic Elements at OxfordPublic Library of Science2014Khatami, AClutterbuck, EThompson, AMcKenna, JPace, DBirks, JSnape, MDPollard, AAIM: We measured meningococcal serogroup C (MenC)-specific memory B-cell responses in infants by Enzyme-Linked Immunospot (ELISpot) following different MenC conjugate vaccine schedules to investigate the impact of priming on immune memory. METHODS: Infants aged 2 months were randomised to receive 1 or 2 doses of MenC-CRM197 at 3 or 3 and 4 months, 1 dose of MenC-TT at 3 months, or no primary MenC doses. All children received a Haemophilus influenzae type b (Hib)-MenC booster at 12 months. Blood was drawn at 5, 12, 12 months +6 days and 13 months of age. RESULTS: Results were available for 110, 103, 76 and 44 children from each group respectively. Following primary immunisations, and prior to the 12-month booster, there were no significant differences between 1- or 2-dose primed children in the number of MenC memory B-cells detected. One month following the booster, children primed with 1 dose MenC-TT had more memory B-cells than children primed with either 1-dose (p = 0.001) or 2-dose (p<0.0001) MenC-CRM197. There were no differences in MenC memory B-cells detected in children who received 1 or 2 doses of MenC-CRM197 in infancy and un-primed children. CONCLUSIONS: MenC-specific memory B-cell production may be more dependent on the type of primary vaccine used than the number of doses administered. Although the mechanistic differences between MenC-CRM197 and MenC-TT priming are unclear, it is possible that structural differences, including the carrier proteins, may underlie differential interactions with B- and T-cell populations, and thus different effects on various memory B-cell subsets. A MenC-TT/Hib-MenC-TT combination for priming/boosting may offer an advantage in inducing more persistent antibody. TRIAL REGISTRATION: EU Clinical Trials Register 2009-016579-31 ClinicalTrials.gov NCT01129518. |
| spellingShingle | Khatami, A Clutterbuck, E Thompson, A McKenna, J Pace, D Birks, J Snape, MD Pollard, A Evaluation of the induction of immune memory following infant immunisation with serogroup C Neisseria meningitidis conjugate vaccines - exploratory analyses within a randomised controlled trial |
| title | Evaluation of the induction of immune memory following infant immunisation with serogroup C Neisseria meningitidis conjugate vaccines - exploratory analyses within a randomised controlled trial |
| title_full | Evaluation of the induction of immune memory following infant immunisation with serogroup C Neisseria meningitidis conjugate vaccines - exploratory analyses within a randomised controlled trial |
| title_fullStr | Evaluation of the induction of immune memory following infant immunisation with serogroup C Neisseria meningitidis conjugate vaccines - exploratory analyses within a randomised controlled trial |
| title_full_unstemmed | Evaluation of the induction of immune memory following infant immunisation with serogroup C Neisseria meningitidis conjugate vaccines - exploratory analyses within a randomised controlled trial |
| title_short | Evaluation of the induction of immune memory following infant immunisation with serogroup C Neisseria meningitidis conjugate vaccines - exploratory analyses within a randomised controlled trial |
| title_sort | evaluation of the induction of immune memory following infant immunisation with serogroup c neisseria meningitidis conjugate vaccines exploratory analyses within a randomised controlled trial |
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