Impact of the native-state stability of human lysozyme variants on protein secretion by Pichia pastoris.

Impact of the native-state stability of human lysozyme variants on protein secretion by Pichia pastoris.

We report the secreted expression by Pichia pastoris of two human lysozyme variants F57I and W64R, associated with systemic amyloid disease, and describe their characterization by biophysical methods. Both variants have a substantially decreased thermostability compared with wild-type human lysozyme...

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Dades bibliogràfiques
Autors principals:	Kumita, JR, Johnson, R, Alcocer, M, Dumoulin, M, Holmqvist, F, McCammon, MG, Robinson, C, Archer, D, Dobson, C
Format:	Journal article
Idioma:	English
Publicat:	2006

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