Enhancing resolution and sensitivity of O-17 solid-state NMR through combining double rotation, H-1 decoupling and satellite modulation for biomolecular applications
17O solid-state NMR is a highly sensitive probe of structural detail of organic solids but improvements in sensitivity and resolution are crucial for it to be applied to larger biological molecules. Here it is shown that high resolution (∼1 ppm) and significant signal enhancement can be achieved by...
| Main Authors: | , , , , , , , |
|---|---|
| Formato: | Journal article |
| Idioma: | English |
| Publicado em: |
2006
|
| _version_ | 1826302226528731136 |
|---|---|
| author | Howes, A Anupold, T Lemaitre, V Kukol, A Watts, A Samoson, A Smith, M Dupree, R |
| author_facet | Howes, A Anupold, T Lemaitre, V Kukol, A Watts, A Samoson, A Smith, M Dupree, R |
| author_sort | Howes, A |
| collection | OXFORD |
| description | 17O solid-state NMR is a highly sensitive probe of structural detail of organic solids but improvements in sensitivity and resolution are crucial for it to be applied to larger biological molecules. Here it is shown that high resolution (∼1 ppm) and significant signal enhancement can be achieved by combining 1H decoupled double rotation (DOR), which narrows the lines by a factor of ∼100 compared to conventional magic angle spinning, and manipulation of the satellite transition populations to transfer magnetisation to the central transition, which produces a signal enhancement of ∼2. © 2006 Elsevier B.V. All rights reserved. |
| first_indexed | 2024-03-07T05:44:20Z |
| format | Journal article |
| id | oxford-uuid:e6ae01d8-8dbe-4037-adb1-3aa338cb5098 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:44:20Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | oxford-uuid:e6ae01d8-8dbe-4037-adb1-3aa338cb50982022-03-27T10:32:58ZEnhancing resolution and sensitivity of O-17 solid-state NMR through combining double rotation, H-1 decoupling and satellite modulation for biomolecular applicationsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e6ae01d8-8dbe-4037-adb1-3aa338cb5098EnglishSymplectic Elements at Oxford2006Howes, AAnupold, TLemaitre, VKukol, AWatts, ASamoson, ASmith, MDupree, R17O solid-state NMR is a highly sensitive probe of structural detail of organic solids but improvements in sensitivity and resolution are crucial for it to be applied to larger biological molecules. Here it is shown that high resolution (∼1 ppm) and significant signal enhancement can be achieved by combining 1H decoupled double rotation (DOR), which narrows the lines by a factor of ∼100 compared to conventional magic angle spinning, and manipulation of the satellite transition populations to transfer magnetisation to the central transition, which produces a signal enhancement of ∼2. © 2006 Elsevier B.V. All rights reserved. |
| spellingShingle | Howes, A Anupold, T Lemaitre, V Kukol, A Watts, A Samoson, A Smith, M Dupree, R Enhancing resolution and sensitivity of O-17 solid-state NMR through combining double rotation, H-1 decoupling and satellite modulation for biomolecular applications |
| title | Enhancing resolution and sensitivity of O-17 solid-state NMR through combining double rotation, H-1 decoupling and satellite modulation for biomolecular applications |
| title_full | Enhancing resolution and sensitivity of O-17 solid-state NMR through combining double rotation, H-1 decoupling and satellite modulation for biomolecular applications |
| title_fullStr | Enhancing resolution and sensitivity of O-17 solid-state NMR through combining double rotation, H-1 decoupling and satellite modulation for biomolecular applications |
| title_full_unstemmed | Enhancing resolution and sensitivity of O-17 solid-state NMR through combining double rotation, H-1 decoupling and satellite modulation for biomolecular applications |
| title_short | Enhancing resolution and sensitivity of O-17 solid-state NMR through combining double rotation, H-1 decoupling and satellite modulation for biomolecular applications |
| title_sort | enhancing resolution and sensitivity of o 17 solid state nmr through combining double rotation h 1 decoupling and satellite modulation for biomolecular applications |
| work_keys_str_mv | AT howesa enhancingresolutionandsensitivityofo17solidstatenmrthroughcombiningdoublerotationh1decouplingandsatellitemodulationforbiomolecularapplications AT anupoldt enhancingresolutionandsensitivityofo17solidstatenmrthroughcombiningdoublerotationh1decouplingandsatellitemodulationforbiomolecularapplications AT lemaitrev enhancingresolutionandsensitivityofo17solidstatenmrthroughcombiningdoublerotationh1decouplingandsatellitemodulationforbiomolecularapplications AT kukola enhancingresolutionandsensitivityofo17solidstatenmrthroughcombiningdoublerotationh1decouplingandsatellitemodulationforbiomolecularapplications AT wattsa enhancingresolutionandsensitivityofo17solidstatenmrthroughcombiningdoublerotationh1decouplingandsatellitemodulationforbiomolecularapplications AT samosona enhancingresolutionandsensitivityofo17solidstatenmrthroughcombiningdoublerotationh1decouplingandsatellitemodulationforbiomolecularapplications AT smithm enhancingresolutionandsensitivityofo17solidstatenmrthroughcombiningdoublerotationh1decouplingandsatellitemodulationforbiomolecularapplications AT dupreer enhancingresolutionandsensitivityofo17solidstatenmrthroughcombiningdoublerotationh1decouplingandsatellitemodulationforbiomolecularapplications |