An HLA class I specific monoclonal antibody that fails to bind to all HLA-A antigens.
A monoclonal antibody, MHM.5, specific for HLA class I antigens, bound to lymphocytes of all donors tested and was thought to bind to a monomorphic determinant. When the antibody was used to precipitate 35S methionine labeled HLA class I molecules from lymphoid cells, which were then isoelectric foc...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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1985
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| _version_ | 1797101065706930176 |
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| author | Ellis, SA Taylor, C Hildreth, J Mcmichael, A |
| author_facet | Ellis, SA Taylor, C Hildreth, J Mcmichael, A |
| author_sort | Ellis, SA |
| collection | OXFORD |
| description | A monoclonal antibody, MHM.5, specific for HLA class I antigens, bound to lymphocytes of all donors tested and was thought to bind to a monomorphic determinant. When the antibody was used to precipitate 35S methionine labeled HLA class I molecules from lymphoid cells, which were then isoelectric focused, it was found that the HLA-A1,A2 and A3 antigens were not precipitated. Similarly, MHM.5, which is IgG1, failed to block complement mediated lysis by alloantisera specific for HLA-A1, 2 and 3, and most other HLA-A antigens. HLA-Aw24, A25, and A32, and all other HLA-B and C typing reactions tested were blocked. Thus the antibody binds to an epitope that is lacking on most A antigens, but present on Aw24, A25, A32 and all B and C locus antigens. Comparison of the published amino acid sequences of HLA-A2, A3, Aw24, A28, Cw3, B7, and B40 suggests some possible sites for this epitope. |
| first_indexed | 2024-03-07T05:46:38Z |
| format | Journal article |
| id | oxford-uuid:e770cefa-0381-46a8-83a6-82636f915359 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:46:38Z |
| publishDate | 1985 |
| record_format | dspace |
| spelling | oxford-uuid:e770cefa-0381-46a8-83a6-82636f9153592022-03-27T10:38:38ZAn HLA class I specific monoclonal antibody that fails to bind to all HLA-A antigens.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e770cefa-0381-46a8-83a6-82636f915359EnglishSymplectic Elements at Oxford1985Ellis, SATaylor, CHildreth, JMcmichael, AA monoclonal antibody, MHM.5, specific for HLA class I antigens, bound to lymphocytes of all donors tested and was thought to bind to a monomorphic determinant. When the antibody was used to precipitate 35S methionine labeled HLA class I molecules from lymphoid cells, which were then isoelectric focused, it was found that the HLA-A1,A2 and A3 antigens were not precipitated. Similarly, MHM.5, which is IgG1, failed to block complement mediated lysis by alloantisera specific for HLA-A1, 2 and 3, and most other HLA-A antigens. HLA-Aw24, A25, and A32, and all other HLA-B and C typing reactions tested were blocked. Thus the antibody binds to an epitope that is lacking on most A antigens, but present on Aw24, A25, A32 and all B and C locus antigens. Comparison of the published amino acid sequences of HLA-A2, A3, Aw24, A28, Cw3, B7, and B40 suggests some possible sites for this epitope. |
| spellingShingle | Ellis, SA Taylor, C Hildreth, J Mcmichael, A An HLA class I specific monoclonal antibody that fails to bind to all HLA-A antigens. |
| title | An HLA class I specific monoclonal antibody that fails to bind to all HLA-A antigens. |
| title_full | An HLA class I specific monoclonal antibody that fails to bind to all HLA-A antigens. |
| title_fullStr | An HLA class I specific monoclonal antibody that fails to bind to all HLA-A antigens. |
| title_full_unstemmed | An HLA class I specific monoclonal antibody that fails to bind to all HLA-A antigens. |
| title_short | An HLA class I specific monoclonal antibody that fails to bind to all HLA-A antigens. |
| title_sort | hla class i specific monoclonal antibody that fails to bind to all hla a antigens |
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