A multicenter comparison of MOG-IgG cell-based assays
<p><strong>Objectives</strong> To compares 3 different myelin oligodendrocyte glycoprotein–immunoglobulin G (IgG) cell-based assays (CBAs) from 3 international centers.</p> <p><strong>Methods</strong> Serum samples from 394 patients were as follows: acute di...
| Päätekijät: | , , , , , , , , , , , |
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| Aineistotyyppi: | Journal article |
| Julkaistu: |
American Academy of Neurology
2019
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| _version_ | 1826302397522116608 |
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| author | Waters, P Komorowski, L Woodhall, M Lederer, S Majed, M Fryer, J Mills, J Flanagan, E Irani, S Kunchok, A McKeon, A Pittock, S |
| author_facet | Waters, P Komorowski, L Woodhall, M Lederer, S Majed, M Fryer, J Mills, J Flanagan, E Irani, S Kunchok, A McKeon, A Pittock, S |
| author_sort | Waters, P |
| collection | OXFORD |
| description | <p><strong>Objectives</strong> To compares 3 different myelin oligodendrocyte glycoprotein–immunoglobulin G (IgG) cell-based assays (CBAs) from 3 international centers.</p> <p><strong>Methods</strong> Serum samples from 394 patients were as follows: acute disseminated encephalomyelitis (28), seronegative neuromyelitis optica (27), optic neuritis (21 single, 2 relapsing), and longitudinally extensive (10 single, 3 recurrent). The control samples were from patients with multiple sclerosis (244), hypergammaglobulinemia (42), and other (17). Seropositivity was determined by visual observation on a fluorescence microscope (Euroimmun fixed CBA, Oxford live cell CBA) or flow cytometry (Mayo live cell fluorescence-activated cell sorting assay).</p> <p><strong>Results</strong> Of 25 samples positive by any methodology, 21 were concordant on all 3 assays, 2 were positive at Oxford and Euroimmun, and 2 were positive only at Oxford. Euroimmun, Mayo, and Oxford results were as follows: clinical specificity 98.1%, 99.6%, and 100%; positive predictive values (PPVs) 82.1%, 95.5%, and 100%; and negative predictive values 79.0%, 78.8%, and 79.8%. Of 5 false-positives, 1 was positive at both Euroimmun and Mayo and 4 were positive at Euroimmun alone.</p> <p><strong>Conclusions</strong> Overall, a high degree of agreement was observed across 3 different MOG-IgG CBAs. Both live cell-based methodologies had superior PPVs to the fixed cell assays, indicating that positive results in these assays are more reliable indicators of MOG autoimmune spectrum disorders.</p> |
| first_indexed | 2024-03-07T05:46:56Z |
| format | Journal article |
| id | oxford-uuid:e78708c5-bd86-4239-a09a-45f9abf9f7d2 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T05:46:56Z |
| publishDate | 2019 |
| publisher | American Academy of Neurology |
| record_format | dspace |
| spelling | oxford-uuid:e78708c5-bd86-4239-a09a-45f9abf9f7d22022-03-27T10:39:32ZA multicenter comparison of MOG-IgG cell-based assaysJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e78708c5-bd86-4239-a09a-45f9abf9f7d2Symplectic Elements at OxfordAmerican Academy of Neurology2019Waters, PKomorowski, LWoodhall, MLederer, SMajed, MFryer, JMills, JFlanagan, EIrani, SKunchok, AMcKeon, APittock, S<p><strong>Objectives</strong> To compares 3 different myelin oligodendrocyte glycoprotein–immunoglobulin G (IgG) cell-based assays (CBAs) from 3 international centers.</p> <p><strong>Methods</strong> Serum samples from 394 patients were as follows: acute disseminated encephalomyelitis (28), seronegative neuromyelitis optica (27), optic neuritis (21 single, 2 relapsing), and longitudinally extensive (10 single, 3 recurrent). The control samples were from patients with multiple sclerosis (244), hypergammaglobulinemia (42), and other (17). Seropositivity was determined by visual observation on a fluorescence microscope (Euroimmun fixed CBA, Oxford live cell CBA) or flow cytometry (Mayo live cell fluorescence-activated cell sorting assay).</p> <p><strong>Results</strong> Of 25 samples positive by any methodology, 21 were concordant on all 3 assays, 2 were positive at Oxford and Euroimmun, and 2 were positive only at Oxford. Euroimmun, Mayo, and Oxford results were as follows: clinical specificity 98.1%, 99.6%, and 100%; positive predictive values (PPVs) 82.1%, 95.5%, and 100%; and negative predictive values 79.0%, 78.8%, and 79.8%. Of 5 false-positives, 1 was positive at both Euroimmun and Mayo and 4 were positive at Euroimmun alone.</p> <p><strong>Conclusions</strong> Overall, a high degree of agreement was observed across 3 different MOG-IgG CBAs. Both live cell-based methodologies had superior PPVs to the fixed cell assays, indicating that positive results in these assays are more reliable indicators of MOG autoimmune spectrum disorders.</p> |
| spellingShingle | Waters, P Komorowski, L Woodhall, M Lederer, S Majed, M Fryer, J Mills, J Flanagan, E Irani, S Kunchok, A McKeon, A Pittock, S A multicenter comparison of MOG-IgG cell-based assays |
| title | A multicenter comparison of MOG-IgG cell-based assays |
| title_full | A multicenter comparison of MOG-IgG cell-based assays |
| title_fullStr | A multicenter comparison of MOG-IgG cell-based assays |
| title_full_unstemmed | A multicenter comparison of MOG-IgG cell-based assays |
| title_short | A multicenter comparison of MOG-IgG cell-based assays |
| title_sort | multicenter comparison of mog igg cell based assays |
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