The importance of tryptic-like activity in purified enzyme blends for efficient islet isolation.

The importance of tryptic-like activity in purified enzyme blends for efficient islet isolation.

BACKGROUND: The isolation of islets from the human pancreas critically depends on an efficient enzyme blend. Previous studies have solely focused on the presence of collagenase and neutral protease/thermolysin. Despite improved characterization of these components, the lot-related variability in ef...

Bibliografski detalji
Glavni autori:	Brandhorst, H, Friberg, A, Andersson, H, Felldin, M, Foss, A, Salmela, K, Lundgren, T, Tibell, A, Tufveson, G, Korsgren, O, Brandhorst, D
Format:	Journal article
Jezik:	English
Izdano:	2009

Slični predmeti

Vitacyte collagenase HA: a novel enzyme blend for efficient human islet isolation.
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Large-Scale Comparison of Liberase HI and Collagenase NB1 Utilized for Human Islet Isolation
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No beneficial effect of two-layer storage compared with UW-storage on human islet isolation and transplantation.
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The effect of truncated collagenase class I isomers on human islet isolation outcome.
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Human islet separation utilizing a closed automated purification system.
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Comparison of neutral proteases and collagenase class I as essential enzymes for human islet isolation
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Degraded collagenase deteriorates islet viability.
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Perfluorohexyloctane improves long-term storage of rat pancreata for subsequent islet isolation.
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New class of oxygen carriers improves islet isolation from long-term stored rat pancreata.
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Experience with human islet transplantation in Sweden.
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Effect of etanercept concentration on human islet integrity
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High concentrations of Etanercept reduce human islet function and integrity
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High Concentrations of Etanercept Reduce Human Islet Function and Integrity
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Quality of isolated pig islets is improved using perfluorohexyloctane for pancreas storage in a split lobe model.
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Quality of Isolated Pig Islets is Improved Using Perfluorohexyloctane for Pancreas Storage in a Split Lobe Model
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