Cdk1 phosphorylates Drosophila Sas-4 to recruit Polo to daughter centrioles and convert them to centrosomes
<p>Centrosomes and cilia are organized by a centriole pa ir comprising an older mother and a younger daughter. Centriole numbers are tightly regulated and daughter centrioles (that assemble in S-phase) cannot themselves duplicate or organise centrosomes until th...
| Main Authors: | , , , |
|---|---|
| Format: | Journal article |
| Published: |
Cell Press
2016
|
| _version_ | 1797101244938977280 |
|---|---|
| author | Raff, J Novak, Z Wainman, A Gartenmann, L |
| author_facet | Raff, J Novak, Z Wainman, A Gartenmann, L |
| author_sort | Raff, J |
| collection | OXFORD |
| description | <p>Centrosomes and cilia are organized by a centriole pa ir comprising an older mother and a younger daughter. Centriole numbers are tightly regulated and daughter centrioles (that assemble in S-phase) cannot themselves duplicate or organise centrosomes until they have passed through mitosis. It is unclear how this mitotic “centriole conversion” is regulated, but it requires Plk1/Polo kinase. Here we show that in flies Cdk1 phosphorylates the conserved centriole protein Sas- 4 during mitosis. This creates a Polo-docking site that helps recruit Polo to daughter centrioles, and is required for the subsequent recruitment of Asterless (Asl) — a protein essential for centriole duplication and mitotic centrosome assembly. Point mutations in Sas-4 th at prevent Cdk1 phosphorylation or Polo - docking do not block centriole disengagement during mitosis , but block efficient centriole conversion and lead to embryonic lethality. These observations can explain why daughter centrioles have to pass through mitosis before they can duplicate and organise a centrosome.</p> |
| first_indexed | 2024-03-07T05:49:04Z |
| format | Journal article |
| id | oxford-uuid:e838561b-941f-4774-a10a-5bf44e57b378 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T05:49:04Z |
| publishDate | 2016 |
| publisher | Cell Press |
| record_format | dspace |
| spelling | oxford-uuid:e838561b-941f-4774-a10a-5bf44e57b3782022-03-27T10:45:06ZCdk1 phosphorylates Drosophila Sas-4 to recruit Polo to daughter centrioles and convert them to centrosomesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e838561b-941f-4774-a10a-5bf44e57b378Symplectic Elements at OxfordCell Press2016Raff, JNovak, ZWainman, AGartenmann, L<p>Centrosomes and cilia are organized by a centriole pa ir comprising an older mother and a younger daughter. Centriole numbers are tightly regulated and daughter centrioles (that assemble in S-phase) cannot themselves duplicate or organise centrosomes until they have passed through mitosis. It is unclear how this mitotic “centriole conversion” is regulated, but it requires Plk1/Polo kinase. Here we show that in flies Cdk1 phosphorylates the conserved centriole protein Sas- 4 during mitosis. This creates a Polo-docking site that helps recruit Polo to daughter centrioles, and is required for the subsequent recruitment of Asterless (Asl) — a protein essential for centriole duplication and mitotic centrosome assembly. Point mutations in Sas-4 th at prevent Cdk1 phosphorylation or Polo - docking do not block centriole disengagement during mitosis , but block efficient centriole conversion and lead to embryonic lethality. These observations can explain why daughter centrioles have to pass through mitosis before they can duplicate and organise a centrosome.</p> |
| spellingShingle | Raff, J Novak, Z Wainman, A Gartenmann, L Cdk1 phosphorylates Drosophila Sas-4 to recruit Polo to daughter centrioles and convert them to centrosomes |
| title | Cdk1 phosphorylates Drosophila Sas-4 to recruit Polo to daughter centrioles and convert them to centrosomes |
| title_full | Cdk1 phosphorylates Drosophila Sas-4 to recruit Polo to daughter centrioles and convert them to centrosomes |
| title_fullStr | Cdk1 phosphorylates Drosophila Sas-4 to recruit Polo to daughter centrioles and convert them to centrosomes |
| title_full_unstemmed | Cdk1 phosphorylates Drosophila Sas-4 to recruit Polo to daughter centrioles and convert them to centrosomes |
| title_short | Cdk1 phosphorylates Drosophila Sas-4 to recruit Polo to daughter centrioles and convert them to centrosomes |
| title_sort | cdk1 phosphorylates drosophila sas 4 to recruit polo to daughter centrioles and convert them to centrosomes |
| work_keys_str_mv | AT raffj cdk1phosphorylatesdrosophilasas4torecruitpolotodaughtercentriolesandconvertthemtocentrosomes AT novakz cdk1phosphorylatesdrosophilasas4torecruitpolotodaughtercentriolesandconvertthemtocentrosomes AT wainmana cdk1phosphorylatesdrosophilasas4torecruitpolotodaughtercentriolesandconvertthemtocentrosomes AT gartenmannl cdk1phosphorylatesdrosophilasas4torecruitpolotodaughtercentriolesandconvertthemtocentrosomes |