Putting proteomics on target: activity-based profiling of ubiquitin and ubiquitin-like processing enzymes.
Modification of proteins with ubiquitin (Ub) and Ub-like modifiers (Ubls) plays a fundamental role in cell biology. As a consequence, proteomics-based efforts were developed to characterize proteins that are modified by Ub or Ubls. A more focused functional proteomics strategy relies on active-site...
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| Format: | Journal article |
| Language: | English |
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2006
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| _version_ | 1826302560736116736 |
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| author | Kessler, B |
| author_facet | Kessler, B |
| author_sort | Kessler, B |
| collection | OXFORD |
| description | Modification of proteins with ubiquitin (Ub) and Ub-like modifiers (Ubls) plays a fundamental role in cell biology. As a consequence, proteomics-based efforts were developed to characterize proteins that are modified by Ub or Ubls. A more focused functional proteomics strategy relies on active-site probes based on the Ub/Ubl scaffold, which specifically targets Ub/Ubl-processing enzymes. Activity-based profiling with such tools led to the identification of novel gene products with Ub/Ubl-processing activity and uncovered novel control mechanisms regulating their activity. This review discusses recent advances in chemistry-based functional proteomics applications, and how this information can provide a framework for drug development against Ub/Ubl-processing enzymes. |
| first_indexed | 2024-03-07T05:49:26Z |
| format | Journal article |
| id | oxford-uuid:e85c0f27-6055-4b2f-aab4-a0c57ca8e7b5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:49:26Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | oxford-uuid:e85c0f27-6055-4b2f-aab4-a0c57ca8e7b52022-03-27T10:45:56ZPutting proteomics on target: activity-based profiling of ubiquitin and ubiquitin-like processing enzymes.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e85c0f27-6055-4b2f-aab4-a0c57ca8e7b5EnglishSymplectic Elements at Oxford2006Kessler, BModification of proteins with ubiquitin (Ub) and Ub-like modifiers (Ubls) plays a fundamental role in cell biology. As a consequence, proteomics-based efforts were developed to characterize proteins that are modified by Ub or Ubls. A more focused functional proteomics strategy relies on active-site probes based on the Ub/Ubl scaffold, which specifically targets Ub/Ubl-processing enzymes. Activity-based profiling with such tools led to the identification of novel gene products with Ub/Ubl-processing activity and uncovered novel control mechanisms regulating their activity. This review discusses recent advances in chemistry-based functional proteomics applications, and how this information can provide a framework for drug development against Ub/Ubl-processing enzymes. |
| spellingShingle | Kessler, B Putting proteomics on target: activity-based profiling of ubiquitin and ubiquitin-like processing enzymes. |
| title | Putting proteomics on target: activity-based profiling of ubiquitin and ubiquitin-like processing enzymes. |
| title_full | Putting proteomics on target: activity-based profiling of ubiquitin and ubiquitin-like processing enzymes. |
| title_fullStr | Putting proteomics on target: activity-based profiling of ubiquitin and ubiquitin-like processing enzymes. |
| title_full_unstemmed | Putting proteomics on target: activity-based profiling of ubiquitin and ubiquitin-like processing enzymes. |
| title_short | Putting proteomics on target: activity-based profiling of ubiquitin and ubiquitin-like processing enzymes. |
| title_sort | putting proteomics on target activity based profiling of ubiquitin and ubiquitin like processing enzymes |
| work_keys_str_mv | AT kesslerb puttingproteomicsontargetactivitybasedprofilingofubiquitinandubiquitinlikeprocessingenzymes |