Fluorescence fluctuation spectroscopy in subdiffraction focal volumes.
We establish fluorescence fluctuation spectroscopy (FFS) with nanoscale detection volumes generated by stimulated emission depletion. Our method applies fluorescence correlation spectroscopy and fluorescence intensity distribution analysis to extract molecular information about mobilities and fluore...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2005
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| _version_ | 1797101275192492032 |
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| author | Kastrup, L Blom, H Eggeling, C Hell, S |
| author_facet | Kastrup, L Blom, H Eggeling, C Hell, S |
| author_sort | Kastrup, L |
| collection | OXFORD |
| description | We establish fluorescence fluctuation spectroscopy (FFS) with nanoscale detection volumes generated by stimulated emission depletion. Our method applies fluorescence correlation spectroscopy and fluorescence intensity distribution analysis to extract molecular information about mobilities and fluorescence emission in solution. The combination of correlation analysis with that of photon intensity distributions reveals a fivefold squeezing of the detection volume over current diffraction-limited systems, which is in full agreement with the simultaneously demonstrated 25-fold reduction in (axial) focal transit time. Our method significantly extends the potential of far-field FFS, including for the noninvasive investigation of molecular reactions at higher concentrations. |
| first_indexed | 2024-03-07T05:49:31Z |
| format | Journal article |
| id | oxford-uuid:e8620d27-1e2d-4c81-a557-44b532cf7cad |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:49:31Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | oxford-uuid:e8620d27-1e2d-4c81-a557-44b532cf7cad2022-03-27T10:46:09ZFluorescence fluctuation spectroscopy in subdiffraction focal volumes.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e8620d27-1e2d-4c81-a557-44b532cf7cadEnglishSymplectic Elements at Oxford2005Kastrup, LBlom, HEggeling, CHell, SWe establish fluorescence fluctuation spectroscopy (FFS) with nanoscale detection volumes generated by stimulated emission depletion. Our method applies fluorescence correlation spectroscopy and fluorescence intensity distribution analysis to extract molecular information about mobilities and fluorescence emission in solution. The combination of correlation analysis with that of photon intensity distributions reveals a fivefold squeezing of the detection volume over current diffraction-limited systems, which is in full agreement with the simultaneously demonstrated 25-fold reduction in (axial) focal transit time. Our method significantly extends the potential of far-field FFS, including for the noninvasive investigation of molecular reactions at higher concentrations. |
| spellingShingle | Kastrup, L Blom, H Eggeling, C Hell, S Fluorescence fluctuation spectroscopy in subdiffraction focal volumes. |
| title | Fluorescence fluctuation spectroscopy in subdiffraction focal volumes. |
| title_full | Fluorescence fluctuation spectroscopy in subdiffraction focal volumes. |
| title_fullStr | Fluorescence fluctuation spectroscopy in subdiffraction focal volumes. |
| title_full_unstemmed | Fluorescence fluctuation spectroscopy in subdiffraction focal volumes. |
| title_short | Fluorescence fluctuation spectroscopy in subdiffraction focal volumes. |
| title_sort | fluorescence fluctuation spectroscopy in subdiffraction focal volumes |
| work_keys_str_mv | AT kastrupl fluorescencefluctuationspectroscopyinsubdiffractionfocalvolumes AT blomh fluorescencefluctuationspectroscopyinsubdiffractionfocalvolumes AT eggelingc fluorescencefluctuationspectroscopyinsubdiffractionfocalvolumes AT hells fluorescencefluctuationspectroscopyinsubdiffractionfocalvolumes |