Calcium and pancreatic beta-cell function. XI. Modification of 45Ca fluxes by Na+ removal.
Pancreatic islets were isolated from noninbred ob/ob mice and used for evaluating how removal of extracellular Na+ affects 45Ca fluxes in the insulin-producing β cells. Isoosmotic replacement of Na+ with Li+ was less efficient in stimulating the incorporation of intracellular (La3+ nondisplaceable)...
| मुख्य लेखकों: | , , , |
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| स्वरूप: | Journal article |
| भाषा: | English |
| प्रकाशित: |
1980
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| _version_ | 1826302571670667264 |
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| author | Hellman, B Andersson, T Berggren, P Rorsman, P |
| author_facet | Hellman, B Andersson, T Berggren, P Rorsman, P |
| author_sort | Hellman, B |
| collection | OXFORD |
| description | Pancreatic islets were isolated from noninbred ob/ob mice and used for evaluating how removal of extracellular Na+ affects 45Ca fluxes in the insulin-producing β cells. Isoosmotic replacement of Na+ with Li+ was less efficient in stimulating the incorporation of intracellular (La3+ nondisplaceable) 45Ca than substitution with choline+ or sucrose. Ample support for the significance of Na+-Ca2+ exchange for the net efflux of Ca2+ was obtained with the observation that Na+ deprivation markedly inhibited the 45Ca washout in a Ca2+-deficient medium. The inhibitory action of Na+ deficiency exceeded that obtained with 20 mM glucose. The 45Ca incorporated after removal of Na+ differed from that taken in response to glucose in not being exchanged with the Ca2+ entering the β cells during glucose stimulation and in appearing also in the postmicrosomal supernatant obtained by differential centrifugation. |
| first_indexed | 2024-03-07T05:49:36Z |
| format | Journal article |
| id | oxford-uuid:e86a6374-82a2-4ab4-bd2b-0994d71f6345 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:49:36Z |
| publishDate | 1980 |
| record_format | dspace |
| spelling | oxford-uuid:e86a6374-82a2-4ab4-bd2b-0994d71f63452022-03-27T10:46:30ZCalcium and pancreatic beta-cell function. XI. Modification of 45Ca fluxes by Na+ removal.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e86a6374-82a2-4ab4-bd2b-0994d71f6345EnglishSymplectic Elements at Oxford1980Hellman, BAndersson, TBerggren, PRorsman, PPancreatic islets were isolated from noninbred ob/ob mice and used for evaluating how removal of extracellular Na+ affects 45Ca fluxes in the insulin-producing β cells. Isoosmotic replacement of Na+ with Li+ was less efficient in stimulating the incorporation of intracellular (La3+ nondisplaceable) 45Ca than substitution with choline+ or sucrose. Ample support for the significance of Na+-Ca2+ exchange for the net efflux of Ca2+ was obtained with the observation that Na+ deprivation markedly inhibited the 45Ca washout in a Ca2+-deficient medium. The inhibitory action of Na+ deficiency exceeded that obtained with 20 mM glucose. The 45Ca incorporated after removal of Na+ differed from that taken in response to glucose in not being exchanged with the Ca2+ entering the β cells during glucose stimulation and in appearing also in the postmicrosomal supernatant obtained by differential centrifugation. |
| spellingShingle | Hellman, B Andersson, T Berggren, P Rorsman, P Calcium and pancreatic beta-cell function. XI. Modification of 45Ca fluxes by Na+ removal. |
| title | Calcium and pancreatic beta-cell function. XI. Modification of 45Ca fluxes by Na+ removal. |
| title_full | Calcium and pancreatic beta-cell function. XI. Modification of 45Ca fluxes by Na+ removal. |
| title_fullStr | Calcium and pancreatic beta-cell function. XI. Modification of 45Ca fluxes by Na+ removal. |
| title_full_unstemmed | Calcium and pancreatic beta-cell function. XI. Modification of 45Ca fluxes by Na+ removal. |
| title_short | Calcium and pancreatic beta-cell function. XI. Modification of 45Ca fluxes by Na+ removal. |
| title_sort | calcium and pancreatic beta cell function xi modification of 45ca fluxes by na removal |
| work_keys_str_mv | AT hellmanb calciumandpancreaticbetacellfunctionximodificationof45cafluxesbynaremoval AT anderssont calciumandpancreaticbetacellfunctionximodificationof45cafluxesbynaremoval AT berggrenp calciumandpancreaticbetacellfunctionximodificationof45cafluxesbynaremoval AT rorsmanp calciumandpancreaticbetacellfunctionximodificationof45cafluxesbynaremoval |