Two-dimensional protein map of human vastus lateralis muscle.
Protein samples of human vastus lateralis muscle were analyzed by two-dimensional gel electrophoresis, using immobilized pH gradients encompassing several pH regions in the first dimension and sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the second dimension. More than 500 protein sp...
| Váldodahkkit: | , , , , |
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| Materiálatiipa: | Journal article |
| Giella: | English |
| Almmustuhtton: |
2003
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| _version_ | 1826302575409889280 |
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| author | Gelfi, C De Palma, S Cerretelli, P Begum, S Wait, R Begum, S |
| author_facet | Gelfi, C De Palma, S Cerretelli, P Begum, S Wait, R Begum, S |
| author_sort | Gelfi, C |
| collection | OXFORD |
| description | Protein samples of human vastus lateralis muscle were analyzed by two-dimensional gel electrophoresis, using immobilized pH gradients encompassing several pH regions in the first dimension and sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the second dimension. More than 500 protein spots on each gel were detected by silver staining, of which 150 were excised, digested in-gel with trypsin and characterized by matrix assisted laser desorptioin/ionization-mass spectrometry and tandem electrospray mass spectrometry. Using these techniques, 124 spots including contractile proteins and metabolic enzymes were identified. This database should provide a valuable resource for the definition of the functional properties of muscle fibres and for assessment of the influence of e.g., hypoxia, ischaemia and ageing on protein level. |
| first_indexed | 2024-03-07T05:49:40Z |
| format | Journal article |
| id | oxford-uuid:e86da0d3-98a9-430a-b220-aeca857650d9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:49:40Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | oxford-uuid:e86da0d3-98a9-430a-b220-aeca857650d92022-03-27T10:46:36ZTwo-dimensional protein map of human vastus lateralis muscle.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e86da0d3-98a9-430a-b220-aeca857650d9EnglishSymplectic Elements at Oxford2003Gelfi, CDe Palma, SCerretelli, PBegum, SWait, RBegum, SProtein samples of human vastus lateralis muscle were analyzed by two-dimensional gel electrophoresis, using immobilized pH gradients encompassing several pH regions in the first dimension and sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the second dimension. More than 500 protein spots on each gel were detected by silver staining, of which 150 were excised, digested in-gel with trypsin and characterized by matrix assisted laser desorptioin/ionization-mass spectrometry and tandem electrospray mass spectrometry. Using these techniques, 124 spots including contractile proteins and metabolic enzymes were identified. This database should provide a valuable resource for the definition of the functional properties of muscle fibres and for assessment of the influence of e.g., hypoxia, ischaemia and ageing on protein level. |
| spellingShingle | Gelfi, C De Palma, S Cerretelli, P Begum, S Wait, R Begum, S Two-dimensional protein map of human vastus lateralis muscle. |
| title | Two-dimensional protein map of human vastus lateralis muscle. |
| title_full | Two-dimensional protein map of human vastus lateralis muscle. |
| title_fullStr | Two-dimensional protein map of human vastus lateralis muscle. |
| title_full_unstemmed | Two-dimensional protein map of human vastus lateralis muscle. |
| title_short | Two-dimensional protein map of human vastus lateralis muscle. |
| title_sort | two dimensional protein map of human vastus lateralis muscle |
| work_keys_str_mv | AT gelfic twodimensionalproteinmapofhumanvastuslateralismuscle AT depalmas twodimensionalproteinmapofhumanvastuslateralismuscle AT cerretellip twodimensionalproteinmapofhumanvastuslateralismuscle AT begums twodimensionalproteinmapofhumanvastuslateralismuscle AT waitr twodimensionalproteinmapofhumanvastuslateralismuscle AT begums twodimensionalproteinmapofhumanvastuslateralismuscle |