Development and optimisation of three-dimensional freeze-dried collagen-based scaffolds
<p>Three-dimensional collagen/chitosan scaffolds fabricated by freeze-drying technique in 96-well polystyrene and PDMS plates were optimized during this study. Surface tension is, by and large, one of the most limiting factors in fabricating freeze-dried scaffolds in small format well plates....
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| 格式: | Thesis |
| 語言: | English |
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2014
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| _version_ | 1826302698506420224 |
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| author | Xue, B Bin Xue |
| author2 | Cui, Z |
| author_facet | Cui, Z Xue, B Bin Xue |
| author_sort | Xue, B |
| collection | OXFORD |
| description | <p>Three-dimensional collagen/chitosan scaffolds fabricated by freeze-drying technique in 96-well polystyrene and PDMS plates were optimized during this study. Surface tension is, by and large, one of the most limiting factors in fabricating freeze-dried scaffolds in small format well plates. Traditionally, bowl-shaped top surfaces of collagen/chitosan scaffolds were common in polystyrene 96-well plate; whereas for PDMS 96-well plate, dome-shaped surfaces were formed. These surface tension phenomena are not desirable in cell studies especially during initial cell seeding. A combination of surface treatment and change of freeze-drying regime were developed to mitigate the surface tension problem in PS and PDMS 96-well plates respectively. Collagen/chitosan scaffolds of varying concentration and composition were experimented in both polystyrene and PDMS 96-well plates. Thin water film treatment with UV cross-linking was successfully used to eliminate meniscus in PS well plates; pre-cooling, on the other hand, was utilised to treat scaffold solutions in PDMS well plates. The resultant matrices all had flat top surfaces and average thickness of 1 mm. As expected, scaffolds with lower overall polymer concentration or, from a compositional perspective, scaffolds with high chitosan content generally had larger pores. Microscopic observation by multi-photon microscope was performed and chemical analyses were conducted to characterize the surface-treated scaffolds. In addition, scaffolds were tested <em>in vitro</em> using DLD-1 cells, hMSCs and fibroblasts for their biological performance. The purpose of this study was to address the problem of using small format culture wells for the fabrication of freeze-dried collagen-based scaffolds for studies of cell growth in 3D culture and in microfluidic perfusion bioreactors.</p> |
| first_indexed | 2024-03-07T05:51:30Z |
| format | Thesis |
| id | oxford-uuid:e90a8ec1-1f1b-4427-858d-0b8f16e62be0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:51:30Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | oxford-uuid:e90a8ec1-1f1b-4427-858d-0b8f16e62be02022-03-27T10:51:20ZDevelopment and optimisation of three-dimensional freeze-dried collagen-based scaffoldsThesishttp://purl.org/coar/resource_type/c_bdccuuid:e90a8ec1-1f1b-4427-858d-0b8f16e62be0Biomedical engineeringEnglishOxford University Research Archive - Valet2014Xue, BBin XueCui, Z<p>Three-dimensional collagen/chitosan scaffolds fabricated by freeze-drying technique in 96-well polystyrene and PDMS plates were optimized during this study. Surface tension is, by and large, one of the most limiting factors in fabricating freeze-dried scaffolds in small format well plates. Traditionally, bowl-shaped top surfaces of collagen/chitosan scaffolds were common in polystyrene 96-well plate; whereas for PDMS 96-well plate, dome-shaped surfaces were formed. These surface tension phenomena are not desirable in cell studies especially during initial cell seeding. A combination of surface treatment and change of freeze-drying regime were developed to mitigate the surface tension problem in PS and PDMS 96-well plates respectively. Collagen/chitosan scaffolds of varying concentration and composition were experimented in both polystyrene and PDMS 96-well plates. Thin water film treatment with UV cross-linking was successfully used to eliminate meniscus in PS well plates; pre-cooling, on the other hand, was utilised to treat scaffold solutions in PDMS well plates. The resultant matrices all had flat top surfaces and average thickness of 1 mm. As expected, scaffolds with lower overall polymer concentration or, from a compositional perspective, scaffolds with high chitosan content generally had larger pores. Microscopic observation by multi-photon microscope was performed and chemical analyses were conducted to characterize the surface-treated scaffolds. In addition, scaffolds were tested <em>in vitro</em> using DLD-1 cells, hMSCs and fibroblasts for their biological performance. The purpose of this study was to address the problem of using small format culture wells for the fabrication of freeze-dried collagen-based scaffolds for studies of cell growth in 3D culture and in microfluidic perfusion bioreactors.</p> |
| spellingShingle | Biomedical engineering Xue, B Bin Xue Development and optimisation of three-dimensional freeze-dried collagen-based scaffolds |
| title | Development and optimisation of three-dimensional freeze-dried collagen-based scaffolds |
| title_full | Development and optimisation of three-dimensional freeze-dried collagen-based scaffolds |
| title_fullStr | Development and optimisation of three-dimensional freeze-dried collagen-based scaffolds |
| title_full_unstemmed | Development and optimisation of three-dimensional freeze-dried collagen-based scaffolds |
| title_short | Development and optimisation of three-dimensional freeze-dried collagen-based scaffolds |
| title_sort | development and optimisation of three dimensional freeze dried collagen based scaffolds |
| topic | Biomedical engineering |
| work_keys_str_mv | AT xueb developmentandoptimisationofthreedimensionalfreezedriedcollagenbasedscaffolds AT binxue developmentandoptimisationofthreedimensionalfreezedriedcollagenbasedscaffolds |