Dissection of a quantitative trait locus for genetic hypertension on rat chromosome 10.
We have previously identified a locus on rat chromosome 10 as carrying a major hypertension gene, BP/SP-1. The 100:1 odds support interval for this gene extended over a 35-centimorgan (cM) region of the chromosome that included the angiotensin I-converting enzyme (ACE) locus as demonstrated in a cro...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1995
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| _version_ | 1826302720772931584 |
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| author | Kreutz, R Hübner, N James, MR Bihoreau, M Gauguier, D Lathrop, G Ganten, D Lindpaintner, K |
| author_facet | Kreutz, R Hübner, N James, MR Bihoreau, M Gauguier, D Lathrop, G Ganten, D Lindpaintner, K |
| author_sort | Kreutz, R |
| collection | OXFORD |
| description | We have previously identified a locus on rat chromosome 10 as carrying a major hypertension gene, BP/SP-1. The 100:1 odds support interval for this gene extended over a 35-centimorgan (cM) region of the chromosome that included the angiotensin I-converting enzyme (ACE) locus as demonstrated in a cross between the stroke-prone spontaneously hypertensive rat (SHRSPHD) and the normotensive Wistar-Kyoto (WKY-0HD) rat. Here we report on the further characterization of BP/SP-1, using a congenic strain, WKY-1HD. WKY-1HD animals carry a 6-cM chromosomal fragment genotypically identical with SHRSPHD on chromosome 10, 26 cM away from the ACE locus. Higher blood pressures in the WKY-1HD strain compared with the WKY-0HD strain, as well as absence of linkage of the chromosome 10 region to blood pressure in an F2 (WKY-1HD x SHRSPHD) population suggested the existence of a quantitative trait locus, termed BP/SP-1a, that lies within the SHRSP-congenic region in WKY-1HD. Linkage analysis in the F2 (WKY-0HD x SHRSPHD) cross revealed that BP/SP-1a is linked to basal blood pressure, whereas a second locus on chromosome 10, termed BP/SP-1b, that maps closer to the ACE locus cosegregates predominantly with blood pressure after exposure to excess dietary NaCl. Thus, we hypothesize that the previously reported effect of BP/SP-1 represents a composite phenotype that can be dissected into at least two specific components on the basis of linkage data and congenic experimentation. One of the loci identified, BP/SP-1a, represents the most precisely mapped locus affecting blood pressure that has so far been characterized by random-marker genome screening. |
| first_indexed | 2024-03-07T05:51:50Z |
| format | Journal article |
| id | oxford-uuid:e92724ae-b655-448b-8447-5e6119e3694d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:51:50Z |
| publishDate | 1995 |
| record_format | dspace |
| spelling | oxford-uuid:e92724ae-b655-448b-8447-5e6119e3694d2022-03-27T10:52:10ZDissection of a quantitative trait locus for genetic hypertension on rat chromosome 10.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e92724ae-b655-448b-8447-5e6119e3694dEnglishSymplectic Elements at Oxford1995Kreutz, RHübner, NJames, MRBihoreau, MGauguier, DLathrop, GGanten, DLindpaintner, KWe have previously identified a locus on rat chromosome 10 as carrying a major hypertension gene, BP/SP-1. The 100:1 odds support interval for this gene extended over a 35-centimorgan (cM) region of the chromosome that included the angiotensin I-converting enzyme (ACE) locus as demonstrated in a cross between the stroke-prone spontaneously hypertensive rat (SHRSPHD) and the normotensive Wistar-Kyoto (WKY-0HD) rat. Here we report on the further characterization of BP/SP-1, using a congenic strain, WKY-1HD. WKY-1HD animals carry a 6-cM chromosomal fragment genotypically identical with SHRSPHD on chromosome 10, 26 cM away from the ACE locus. Higher blood pressures in the WKY-1HD strain compared with the WKY-0HD strain, as well as absence of linkage of the chromosome 10 region to blood pressure in an F2 (WKY-1HD x SHRSPHD) population suggested the existence of a quantitative trait locus, termed BP/SP-1a, that lies within the SHRSP-congenic region in WKY-1HD. Linkage analysis in the F2 (WKY-0HD x SHRSPHD) cross revealed that BP/SP-1a is linked to basal blood pressure, whereas a second locus on chromosome 10, termed BP/SP-1b, that maps closer to the ACE locus cosegregates predominantly with blood pressure after exposure to excess dietary NaCl. Thus, we hypothesize that the previously reported effect of BP/SP-1 represents a composite phenotype that can be dissected into at least two specific components on the basis of linkage data and congenic experimentation. One of the loci identified, BP/SP-1a, represents the most precisely mapped locus affecting blood pressure that has so far been characterized by random-marker genome screening. |
| spellingShingle | Kreutz, R Hübner, N James, MR Bihoreau, M Gauguier, D Lathrop, G Ganten, D Lindpaintner, K Dissection of a quantitative trait locus for genetic hypertension on rat chromosome 10. |
| title | Dissection of a quantitative trait locus for genetic hypertension on rat chromosome 10. |
| title_full | Dissection of a quantitative trait locus for genetic hypertension on rat chromosome 10. |
| title_fullStr | Dissection of a quantitative trait locus for genetic hypertension on rat chromosome 10. |
| title_full_unstemmed | Dissection of a quantitative trait locus for genetic hypertension on rat chromosome 10. |
| title_short | Dissection of a quantitative trait locus for genetic hypertension on rat chromosome 10. |
| title_sort | dissection of a quantitative trait locus for genetic hypertension on rat chromosome 10 |
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