Temperature of a dengue rapid diagnostic test under tropical climatic conditions: a follow up study
The Dengue Duo Rapid Diagnostic Test (SD Dengue RDT) has good specificity and sensitivity for dengue diagnosis in rural tropical areas. In a previous study, using four control sera, we demonstrated that that the diagnostic accuracy of these RDTs remains stable after long-term storage at high tempera...
| Prif Awduron: | , , , , , , , , , , |
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| Fformat: | Journal article |
| Iaith: | English |
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Public Library of Science
2017
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| _version_ | 1826302741172977664 |
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| author | Sengvilaipaseuth, O Phommasone, K de Lamballerie, X Vongsouvath, M Phonemixay, O Blacksell, S Mayxay, M Keomany, S Souvannasing, P Newton, P Dubot-Pérès, A |
| author_facet | Sengvilaipaseuth, O Phommasone, K de Lamballerie, X Vongsouvath, M Phonemixay, O Blacksell, S Mayxay, M Keomany, S Souvannasing, P Newton, P Dubot-Pérès, A |
| author_sort | Sengvilaipaseuth, O |
| collection | OXFORD |
| description | The Dengue Duo Rapid Diagnostic Test (SD Dengue RDT) has good specificity and sensitivity for dengue diagnosis in rural tropical areas. In a previous study, using four control sera, we demonstrated that that the diagnostic accuracy of these RDTs remains stable after long-term storage at high temperatures. We extended this study by testing sera from 119 febrile patients collected between July-November 2012 at Salavan Provincial Hospital (southern Laos) with RDTs stored for 6 months at 4°C, 35° and in a hut (miniature traditional house) at Lao ambient temperatures. The dengue NS1 antigen results from RDTs stored at 35°C and in the hut demonstrated 100% agreement with those stored at 4°C. However, lower positive percent agreements, with broad 95%CI, were observed for the tests: IgM, 60% (14.7-94.7) and 40% (5.3-85.3) for RDTs store at 35°C and in the hut, compared to those stored at 4°C, respectively. This study strenghtens the evidence of the robustness of the NS1 antigen detection RDT for the diagnosis of dengue after storage at tropical temperatures. |
| first_indexed | 2024-03-07T05:52:08Z |
| format | Journal article |
| id | oxford-uuid:e942d9e5-84b9-4ff3-a50e-d098b9f40a6c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:52:08Z |
| publishDate | 2017 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | oxford-uuid:e942d9e5-84b9-4ff3-a50e-d098b9f40a6c2022-03-27T10:52:56ZTemperature of a dengue rapid diagnostic test under tropical climatic conditions: a follow up studyJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e942d9e5-84b9-4ff3-a50e-d098b9f40a6cEnglishSymplectic Elements at OxfordPublic Library of Science2017Sengvilaipaseuth, OPhommasone, Kde Lamballerie, XVongsouvath, MPhonemixay, OBlacksell, SMayxay, MKeomany, SSouvannasing, PNewton, PDubot-Pérès, AThe Dengue Duo Rapid Diagnostic Test (SD Dengue RDT) has good specificity and sensitivity for dengue diagnosis in rural tropical areas. In a previous study, using four control sera, we demonstrated that that the diagnostic accuracy of these RDTs remains stable after long-term storage at high temperatures. We extended this study by testing sera from 119 febrile patients collected between July-November 2012 at Salavan Provincial Hospital (southern Laos) with RDTs stored for 6 months at 4°C, 35° and in a hut (miniature traditional house) at Lao ambient temperatures. The dengue NS1 antigen results from RDTs stored at 35°C and in the hut demonstrated 100% agreement with those stored at 4°C. However, lower positive percent agreements, with broad 95%CI, were observed for the tests: IgM, 60% (14.7-94.7) and 40% (5.3-85.3) for RDTs store at 35°C and in the hut, compared to those stored at 4°C, respectively. This study strenghtens the evidence of the robustness of the NS1 antigen detection RDT for the diagnosis of dengue after storage at tropical temperatures. |
| spellingShingle | Sengvilaipaseuth, O Phommasone, K de Lamballerie, X Vongsouvath, M Phonemixay, O Blacksell, S Mayxay, M Keomany, S Souvannasing, P Newton, P Dubot-Pérès, A Temperature of a dengue rapid diagnostic test under tropical climatic conditions: a follow up study |
| title | Temperature of a dengue rapid diagnostic test under tropical climatic conditions: a follow up study |
| title_full | Temperature of a dengue rapid diagnostic test under tropical climatic conditions: a follow up study |
| title_fullStr | Temperature of a dengue rapid diagnostic test under tropical climatic conditions: a follow up study |
| title_full_unstemmed | Temperature of a dengue rapid diagnostic test under tropical climatic conditions: a follow up study |
| title_short | Temperature of a dengue rapid diagnostic test under tropical climatic conditions: a follow up study |
| title_sort | temperature of a dengue rapid diagnostic test under tropical climatic conditions a follow up study |
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