STUDIES ON THE EXCHANGE OF VALINE-OXYGEN DURING THE BIOSYNTHESIS OF DELTA-(L-ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VALINE

STUDIES ON THE EXCHANGE OF VALINE-OXYGEN DURING THE BIOSYNTHESIS OF DELTA-(L-ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VALINE

Incorporation of [4-2H6,18O2]-valine into δ-(L)-α-aminoadipoyl)-L-cysteinyl-D-valine (ACV), by intact cells of Cephalosporium acremonium, demonstrated the intracellular exchange of one and both valine oxygen atoms. Incubation of [18O2]-valine with the purified ACV synthetase from C. acremonium gave...

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Chi tiết về thư mục
Những tác giả chính: Baldwin, J, Adlington, R, Bird, J, Field, R, Ocallaghan, N, Schofield, C
Định dạng: Journal article
Ngôn ngữ:English
Được phát hành: 1992
Miêu tả
Tóm tắt:Incorporation of [4-2H6,18O2]-valine into δ-(L)-α-aminoadipoyl)-L-cysteinyl-D-valine (ACV), by intact cells of Cephalosporium acremonium, demonstrated the intracellular exchange of one and both valine oxygen atoms. Incubation of [18O2]-valine with the purified ACV synthetase from C. acremonium gave exclusive incorporation of a single 18O label into ACV, consistent witheffectively non-reversible formation of a covalent valinoyl-ACV synthetase intermediate under in vitro conditions. © 1992.

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