Creating supported plasma membrane bilayers using acoustic pressure
Model membrane systems are essential tools for the study of biological processes in a simplified setting to reveal the underlying physicochemical principles. As cell-derived membrane systems, giant plasma membrane vesicles (GPMVs) constitute an intermediate model between live cells and fully artific...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
MDPI
2020
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| _version_ | 1797101483570757632 |
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| author | Sezgin, E Carugo, D Levental, I Stride, E Eggeling, C |
| author_facet | Sezgin, E Carugo, D Levental, I Stride, E Eggeling, C |
| author_sort | Sezgin, E |
| collection | OXFORD |
| description | Model membrane systems are essential tools for the study of biological processes in a simplified setting to reveal the underlying physicochemical principles. As cell-derived membrane systems, giant plasma membrane vesicles (GPMVs) constitute an intermediate model between live cells and fully artificial structures. Certain applications, however, require planar membrane surfaces. Here, we report a new approach for creating supported plasma membrane bilayers (SPMBs) by bursting cell-derived GPMVs using ultrasound within a microfluidic device. We show that the mobility of outer leaflet molecules is preserved in SPMBs, suggesting that they are accessible on the surface of the bilayers. Such model membrane systems are potentially useful in many applications requiring detailed characterization of plasma membrane dynamics. |
| first_indexed | 2024-03-07T05:52:36Z |
| format | Journal article |
| id | oxford-uuid:e967529c-daf6-4d7e-af69-f394639d7e81 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:52:36Z |
| publishDate | 2020 |
| publisher | MDPI |
| record_format | dspace |
| spelling | oxford-uuid:e967529c-daf6-4d7e-af69-f394639d7e812022-03-27T10:54:04ZCreating supported plasma membrane bilayers using acoustic pressureJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e967529c-daf6-4d7e-af69-f394639d7e81EnglishSymplectic ElementsMDPI2020Sezgin, ECarugo, DLevental, IStride, EEggeling, CModel membrane systems are essential tools for the study of biological processes in a simplified setting to reveal the underlying physicochemical principles. As cell-derived membrane systems, giant plasma membrane vesicles (GPMVs) constitute an intermediate model between live cells and fully artificial structures. Certain applications, however, require planar membrane surfaces. Here, we report a new approach for creating supported plasma membrane bilayers (SPMBs) by bursting cell-derived GPMVs using ultrasound within a microfluidic device. We show that the mobility of outer leaflet molecules is preserved in SPMBs, suggesting that they are accessible on the surface of the bilayers. Such model membrane systems are potentially useful in many applications requiring detailed characterization of plasma membrane dynamics. |
| spellingShingle | Sezgin, E Carugo, D Levental, I Stride, E Eggeling, C Creating supported plasma membrane bilayers using acoustic pressure |
| title | Creating supported plasma membrane bilayers using acoustic pressure |
| title_full | Creating supported plasma membrane bilayers using acoustic pressure |
| title_fullStr | Creating supported plasma membrane bilayers using acoustic pressure |
| title_full_unstemmed | Creating supported plasma membrane bilayers using acoustic pressure |
| title_short | Creating supported plasma membrane bilayers using acoustic pressure |
| title_sort | creating supported plasma membrane bilayers using acoustic pressure |
| work_keys_str_mv | AT sezgine creatingsupportedplasmamembranebilayersusingacousticpressure AT carugod creatingsupportedplasmamembranebilayersusingacousticpressure AT leventali creatingsupportedplasmamembranebilayersusingacousticpressure AT stridee creatingsupportedplasmamembranebilayersusingacousticpressure AT eggelingc creatingsupportedplasmamembranebilayersusingacousticpressure |