Extracting axial depth and trajectory trend using astigmatism, Gaussian fitting, and CNNs for protein tracking
Accurate analysis of vesicle trafficking in live cells is challenging for a number of reasons: varying appearance, complex protein movement patterns, and imaging conditions. To allow fast image acquisition, we study how employing an astigmatism can be utilized for obtaining additional information th...
Main Authors: | Delas Penas, K, Dmitrieva, M, Lefebvre, J, Zenner, H, Allgeyer, E, Booth, M, St Johnston, D, Rittscher, J |
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Format: | Conference item |
Language: | English |
Published: |
IEEE
2020
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