In vivo evaluation of bacterial infection involving morphologically different surgical meshes.
OBJECTIVE: To study the influence of morphology of surgical meshes on the course of bacterial infection under the influence of the host immune system in an in vivo chronic bacterial infection model. BACKGROUND: The use of prosthetic meshes has increased dramatically the last decades in abdominal wa...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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2010
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| _version_ | 1797101888234061824 |
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| author | Engelsman, A Dam, v van der Mei, H Busscher, H Ploeg, R |
| author_facet | Engelsman, A Dam, v van der Mei, H Busscher, H Ploeg, R |
| author_sort | Engelsman, A |
| collection | OXFORD |
| description | OBJECTIVE: To study the influence of morphology of surgical meshes on the course of bacterial infection under the influence of the host immune system in an in vivo chronic bacterial infection model. BACKGROUND: The use of prosthetic meshes has increased dramatically the last decades in abdominal wall reconstructive surgery. Whereas infection is becoming a more frequent complication, attention is increasingly drawn to the influence of the surgeon's mesh choice on the course of this complication. METHODS: Samples of 6 often applied surgical meshes were contaminated with a bioluminescent strain of Staphylococcus aureus and implanted subcutaneously in an immunocompetent BALB/c mouse. The intensity and the spreading of bioluminescence (ie, p/s/cm/sr) were analyzed non-invasively in vivo during a 10-day follow-up period. RESULTS: Over the course of infection, multifilament polypropylene and hydrophobic materials showed a significantly higher persistence of bacteria as well as spreading of infection compared to all other meshes. In contrast, infection resolved in almost all animals with a low-weight polyester mesh. CONCLUSION: The results of this study are in accordance with circumstantial evidence from limited clinical reports on infection involving surgical meshes and suggest that multifilament and hydrophobic meshes significantly increase bacterial persistence or spreading in the infected area in contrast to monofilament polypropylene and lightweight meshes. Therefore, the surgeon should consider this outcome when choosing a mesh graft for limiting infection in abdominal wall repair. |
| first_indexed | 2024-03-07T05:58:19Z |
| format | Journal article |
| id | oxford-uuid:eb4893d6-680f-4fd7-b7c6-0862f808f031 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:58:19Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:eb4893d6-680f-4fd7-b7c6-0862f808f0312022-03-27T11:08:31ZIn vivo evaluation of bacterial infection involving morphologically different surgical meshes.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:eb4893d6-680f-4fd7-b7c6-0862f808f031EnglishSymplectic Elements at Oxford2010Engelsman, ADam, vvan der Mei, HBusscher, HPloeg, R OBJECTIVE: To study the influence of morphology of surgical meshes on the course of bacterial infection under the influence of the host immune system in an in vivo chronic bacterial infection model. BACKGROUND: The use of prosthetic meshes has increased dramatically the last decades in abdominal wall reconstructive surgery. Whereas infection is becoming a more frequent complication, attention is increasingly drawn to the influence of the surgeon's mesh choice on the course of this complication. METHODS: Samples of 6 often applied surgical meshes were contaminated with a bioluminescent strain of Staphylococcus aureus and implanted subcutaneously in an immunocompetent BALB/c mouse. The intensity and the spreading of bioluminescence (ie, p/s/cm/sr) were analyzed non-invasively in vivo during a 10-day follow-up period. RESULTS: Over the course of infection, multifilament polypropylene and hydrophobic materials showed a significantly higher persistence of bacteria as well as spreading of infection compared to all other meshes. In contrast, infection resolved in almost all animals with a low-weight polyester mesh. CONCLUSION: The results of this study are in accordance with circumstantial evidence from limited clinical reports on infection involving surgical meshes and suggest that multifilament and hydrophobic meshes significantly increase bacterial persistence or spreading in the infected area in contrast to monofilament polypropylene and lightweight meshes. Therefore, the surgeon should consider this outcome when choosing a mesh graft for limiting infection in abdominal wall repair. |
| spellingShingle | Engelsman, A Dam, v van der Mei, H Busscher, H Ploeg, R In vivo evaluation of bacterial infection involving morphologically different surgical meshes. |
| title | In vivo evaluation of bacterial infection involving morphologically different surgical meshes. |
| title_full | In vivo evaluation of bacterial infection involving morphologically different surgical meshes. |
| title_fullStr | In vivo evaluation of bacterial infection involving morphologically different surgical meshes. |
| title_full_unstemmed | In vivo evaluation of bacterial infection involving morphologically different surgical meshes. |
| title_short | In vivo evaluation of bacterial infection involving morphologically different surgical meshes. |
| title_sort | in vivo evaluation of bacterial infection involving morphologically different surgical meshes |
| work_keys_str_mv | AT engelsmana invivoevaluationofbacterialinfectioninvolvingmorphologicallydifferentsurgicalmeshes AT damv invivoevaluationofbacterialinfectioninvolvingmorphologicallydifferentsurgicalmeshes AT vandermeih invivoevaluationofbacterialinfectioninvolvingmorphologicallydifferentsurgicalmeshes AT busscherh invivoevaluationofbacterialinfectioninvolvingmorphologicallydifferentsurgicalmeshes AT ploegr invivoevaluationofbacterialinfectioninvolvingmorphologicallydifferentsurgicalmeshes |