| Summary: | One of the key research areas surrounding HIV-1 concerns the regulation of the fusion event that occurs between the virus particle and the host cell during entry. Even if it is universally accepted that the large GTPase dynamin-2 is important during HIV-1 entry its exact role during the first steps of HIV-1 infection is not well characterized. Here, we utilised a multidisciplinary approach to study the DNM2 role during fusion of HIV-1 in primary resting CD4 T and TZM-bl cells. We have combined advanced light microscopy and functional cell- based assays to experimentally assess the role of dynamin-2 during these processes. Overall our data suggests that dynamin 2, as a tetramer, might help to establish hemi-fusion and stabilizes the pore during HIV-1 fusion.
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