Direct observation of glycans bonded to proteins and lipids at the single-molecule level
Proteins and lipids decorated with glycans are found throughout biological entities, playing roles in biological functions and dysfunctions. Current analytical strategies for these glycan-decorated biomolecules, termed glycoconjugates, rely on ensemble-averaged methods that do not provide a full vie...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
American Association for the Advancement of Science
2023
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| _version_ | 1797111125789114368 |
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| author | Anggara, K Sršan, L Jaroentomeechai, T Wu, X Rauschenbach, S Narimatsu, Y Clausen, H Ziegler, T Miller, RL Kern, K |
| author_facet | Anggara, K Sršan, L Jaroentomeechai, T Wu, X Rauschenbach, S Narimatsu, Y Clausen, H Ziegler, T Miller, RL Kern, K |
| author_sort | Anggara, K |
| collection | OXFORD |
| description | Proteins and lipids decorated with glycans are found throughout biological entities, playing roles in biological functions and dysfunctions. Current analytical strategies for these glycan-decorated biomolecules, termed glycoconjugates, rely on ensemble-averaged methods that do not provide a full view of positions and structures of glycans attached at individual sites in a given molecule, especially for glycoproteins. We show single-molecule analysis of glycoconjugates by direct imaging of individual glycoconjugate molecules using low-temperature scanning tunneling microscopy. Intact glycoconjugate ions from electrospray are soft-landed on a surface for their direct single-molecule imaging. The submolecular imaging resolution corroborated by quantum mechanical modeling unveils whole structures and attachment sites of glycans in glycopeptides, glycolipids, N-glycoproteins, and O-glycoproteins densely decorated with glycans. |
| first_indexed | 2024-03-07T08:05:51Z |
| format | Journal article |
| id | oxford-uuid:eb730070-a5be-494d-9927-27957490122b |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T08:05:51Z |
| publishDate | 2023 |
| publisher | American Association for the Advancement of Science |
| record_format | dspace |
| spelling | oxford-uuid:eb730070-a5be-494d-9927-27957490122b2023-10-23T10:58:48ZDirect observation of glycans bonded to proteins and lipids at the single-molecule levelJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:eb730070-a5be-494d-9927-27957490122bEnglishSymplectic ElementsAmerican Association for the Advancement of Science2023Anggara, KSršan, LJaroentomeechai, TWu, XRauschenbach, SNarimatsu, YClausen, HZiegler, TMiller, RLKern, KProteins and lipids decorated with glycans are found throughout biological entities, playing roles in biological functions and dysfunctions. Current analytical strategies for these glycan-decorated biomolecules, termed glycoconjugates, rely on ensemble-averaged methods that do not provide a full view of positions and structures of glycans attached at individual sites in a given molecule, especially for glycoproteins. We show single-molecule analysis of glycoconjugates by direct imaging of individual glycoconjugate molecules using low-temperature scanning tunneling microscopy. Intact glycoconjugate ions from electrospray are soft-landed on a surface for their direct single-molecule imaging. The submolecular imaging resolution corroborated by quantum mechanical modeling unveils whole structures and attachment sites of glycans in glycopeptides, glycolipids, N-glycoproteins, and O-glycoproteins densely decorated with glycans. |
| spellingShingle | Anggara, K Sršan, L Jaroentomeechai, T Wu, X Rauschenbach, S Narimatsu, Y Clausen, H Ziegler, T Miller, RL Kern, K Direct observation of glycans bonded to proteins and lipids at the single-molecule level |
| title | Direct observation of glycans bonded to proteins and lipids at the single-molecule level |
| title_full | Direct observation of glycans bonded to proteins and lipids at the single-molecule level |
| title_fullStr | Direct observation of glycans bonded to proteins and lipids at the single-molecule level |
| title_full_unstemmed | Direct observation of glycans bonded to proteins and lipids at the single-molecule level |
| title_short | Direct observation of glycans bonded to proteins and lipids at the single-molecule level |
| title_sort | direct observation of glycans bonded to proteins and lipids at the single molecule level |
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