Low-cost, frequency-domain, fluorescence lifetime confocal microscopy.
We describe the theory and implementation of a frequency-domain fluorescence lifetime confocal microscope using switched diode laser illumination. Standard, communications-type, radio-frequency electronics are used to provide inexpensive modulation references and to perform phase-sensitive detection...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2004
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| _version_ | 1797101930668883968 |
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| author | Booth, M Wilson, T |
| author_facet | Booth, M Wilson, T |
| author_sort | Booth, M |
| collection | OXFORD |
| description | We describe the theory and implementation of a frequency-domain fluorescence lifetime confocal microscope using switched diode laser illumination. Standard, communications-type, radio-frequency electronics are used to provide inexpensive modulation references and to perform phase-sensitive detection. This allows the rapid acquisition of fluorescence intensity and lifetime images and their display in real time. We show fluorescence lifetime images of bead objects and fluorescence lifetime images of biological specimens from a single confocal scan. |
| first_indexed | 2024-03-07T05:58:50Z |
| format | Journal article |
| id | oxford-uuid:eb79270f-217d-4bd7-9672-3753e21de11f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:58:50Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:eb79270f-217d-4bd7-9672-3753e21de11f2022-03-27T11:09:53ZLow-cost, frequency-domain, fluorescence lifetime confocal microscopy.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:eb79270f-217d-4bd7-9672-3753e21de11fEnglishSymplectic Elements at Oxford2004Booth, MWilson, TWe describe the theory and implementation of a frequency-domain fluorescence lifetime confocal microscope using switched diode laser illumination. Standard, communications-type, radio-frequency electronics are used to provide inexpensive modulation references and to perform phase-sensitive detection. This allows the rapid acquisition of fluorescence intensity and lifetime images and their display in real time. We show fluorescence lifetime images of bead objects and fluorescence lifetime images of biological specimens from a single confocal scan. |
| spellingShingle | Booth, M Wilson, T Low-cost, frequency-domain, fluorescence lifetime confocal microscopy. |
| title | Low-cost, frequency-domain, fluorescence lifetime confocal microscopy. |
| title_full | Low-cost, frequency-domain, fluorescence lifetime confocal microscopy. |
| title_fullStr | Low-cost, frequency-domain, fluorescence lifetime confocal microscopy. |
| title_full_unstemmed | Low-cost, frequency-domain, fluorescence lifetime confocal microscopy. |
| title_short | Low-cost, frequency-domain, fluorescence lifetime confocal microscopy. |
| title_sort | low cost frequency domain fluorescence lifetime confocal microscopy |
| work_keys_str_mv | AT boothm lowcostfrequencydomainfluorescencelifetimeconfocalmicroscopy AT wilsont lowcostfrequencydomainfluorescencelifetimeconfocalmicroscopy |