Cullin 4-ring finger-ligase plays a key role in the control of endoreplication cycles in Arabidopsis trichomes.
One of the predominant cell-cycle programs found in mature tissues is endoreplication, also known as endoreduplication, that leads to cellular polyploidy. A key question for the understanding of endoreplication cycles is how oscillating levels of cyclin-dependent kinase activity are generated that c...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2010
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| _version_ | 1797101979795718144 |
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| author | Roodbarkelari, F Bramsiepe, J Weinl, C Marquardt, S Novák, B Jakoby, M Lechner, E Genschik, P Schnittger, A |
| author_facet | Roodbarkelari, F Bramsiepe, J Weinl, C Marquardt, S Novák, B Jakoby, M Lechner, E Genschik, P Schnittger, A |
| author_sort | Roodbarkelari, F |
| collection | OXFORD |
| description | One of the predominant cell-cycle programs found in mature tissues is endoreplication, also known as endoreduplication, that leads to cellular polyploidy. A key question for the understanding of endoreplication cycles is how oscillating levels of cyclin-dependent kinase activity are generated that control repeated rounds of DNA replication. The APC/C performs a pivotal function in the mitotic cell cycle by promoting anaphase and paving the road for a new round of DNA replication. However, using marker lines and plants in which APC/C components are knocked down, we show here that outgrowing and endoreplicating Arabidopsis leaf hairs display no or very little APC/C activity. Instead we find that RBX1-containing Cullin-RING E3 ubiquitin-Ligases (CRLs) are of central importance for the progression through endoreplication cycles; in particular, we have identified CULLIN4 as a major regulator of endoreplication in Arabidopsis trichomes. We have incorporated our findings into a bio-mathematical simulation presenting a robust two-step model of endoreplication control with one type of cyclin-dependent kinase inhibitor function for entry and a CRL-dependent oscillation of cyclin-dependent kinase activity via degradation of a second type of CDK inhibitor during endoreplication cycles. |
| first_indexed | 2024-03-07T05:59:27Z |
| format | Journal article |
| id | oxford-uuid:eba9e4f7-ad61-4363-8100-74f9f04d9ed0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:59:27Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:eba9e4f7-ad61-4363-8100-74f9f04d9ed02022-03-27T11:11:31ZCullin 4-ring finger-ligase plays a key role in the control of endoreplication cycles in Arabidopsis trichomes.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:eba9e4f7-ad61-4363-8100-74f9f04d9ed0EnglishSymplectic Elements at Oxford2010Roodbarkelari, FBramsiepe, JWeinl, CMarquardt, SNovák, BJakoby, MLechner, EGenschik, PSchnittger, AOne of the predominant cell-cycle programs found in mature tissues is endoreplication, also known as endoreduplication, that leads to cellular polyploidy. A key question for the understanding of endoreplication cycles is how oscillating levels of cyclin-dependent kinase activity are generated that control repeated rounds of DNA replication. The APC/C performs a pivotal function in the mitotic cell cycle by promoting anaphase and paving the road for a new round of DNA replication. However, using marker lines and plants in which APC/C components are knocked down, we show here that outgrowing and endoreplicating Arabidopsis leaf hairs display no or very little APC/C activity. Instead we find that RBX1-containing Cullin-RING E3 ubiquitin-Ligases (CRLs) are of central importance for the progression through endoreplication cycles; in particular, we have identified CULLIN4 as a major regulator of endoreplication in Arabidopsis trichomes. We have incorporated our findings into a bio-mathematical simulation presenting a robust two-step model of endoreplication control with one type of cyclin-dependent kinase inhibitor function for entry and a CRL-dependent oscillation of cyclin-dependent kinase activity via degradation of a second type of CDK inhibitor during endoreplication cycles. |
| spellingShingle | Roodbarkelari, F Bramsiepe, J Weinl, C Marquardt, S Novák, B Jakoby, M Lechner, E Genschik, P Schnittger, A Cullin 4-ring finger-ligase plays a key role in the control of endoreplication cycles in Arabidopsis trichomes. |
| title | Cullin 4-ring finger-ligase plays a key role in the control of endoreplication cycles in Arabidopsis trichomes. |
| title_full | Cullin 4-ring finger-ligase plays a key role in the control of endoreplication cycles in Arabidopsis trichomes. |
| title_fullStr | Cullin 4-ring finger-ligase plays a key role in the control of endoreplication cycles in Arabidopsis trichomes. |
| title_full_unstemmed | Cullin 4-ring finger-ligase plays a key role in the control of endoreplication cycles in Arabidopsis trichomes. |
| title_short | Cullin 4-ring finger-ligase plays a key role in the control of endoreplication cycles in Arabidopsis trichomes. |
| title_sort | cullin 4 ring finger ligase plays a key role in the control of endoreplication cycles in arabidopsis trichomes |
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