CpG islands recruit a histone H3 lysine 36 demethylase.
In higher eukaryotes, up to 70% of genes have high levels of nonmethylated cytosine/guanine base pairs (CpGs) surrounding promoters and gene regulatory units. These features, called CpG islands, were identified over 20 years ago, but there remains little mechanistic evidence to suggest how these eni...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2010
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| _version_ | 1797101991673987072 |
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| author | Blackledge, N Zhou, J Tolstorukov, M Farcas, A Park, P Klose, R |
| author_facet | Blackledge, N Zhou, J Tolstorukov, M Farcas, A Park, P Klose, R |
| author_sort | Blackledge, N |
| collection | OXFORD |
| description | In higher eukaryotes, up to 70% of genes have high levels of nonmethylated cytosine/guanine base pairs (CpGs) surrounding promoters and gene regulatory units. These features, called CpG islands, were identified over 20 years ago, but there remains little mechanistic evidence to suggest how these enigmatic elements contribute to promoter function, except that they are refractory to epigenetic silencing by DNA methylation. Here we show that CpG islands directly recruit the H3K36-specific lysine demethylase enzyme KDM2A. Nucleation of KDM2A at these elements results in removal of H3K36 methylation, creating CpG island chromatin that is uniquely depleted of this modification. KDM2A utilizes a zinc finger CxxC (ZF-CxxC) domain that preferentially recognizes nonmethylated CpG DNA, and binding is blocked when the CpG DNA is methylated, thus constraining KDM2A to nonmethylated CpG islands. These data expose a straightforward mechanism through which KDM2A delineates a unique architecture that differentiates CpG island chromatin from bulk chromatin. |
| first_indexed | 2024-03-07T05:59:38Z |
| format | Journal article |
| id | oxford-uuid:ebba3c4d-1020-4454-8868-82e1bb272180 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:59:38Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:ebba3c4d-1020-4454-8868-82e1bb2721802022-03-27T11:12:01ZCpG islands recruit a histone H3 lysine 36 demethylase.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ebba3c4d-1020-4454-8868-82e1bb272180EnglishSymplectic Elements at Oxford2010Blackledge, NZhou, JTolstorukov, MFarcas, APark, PKlose, RIn higher eukaryotes, up to 70% of genes have high levels of nonmethylated cytosine/guanine base pairs (CpGs) surrounding promoters and gene regulatory units. These features, called CpG islands, were identified over 20 years ago, but there remains little mechanistic evidence to suggest how these enigmatic elements contribute to promoter function, except that they are refractory to epigenetic silencing by DNA methylation. Here we show that CpG islands directly recruit the H3K36-specific lysine demethylase enzyme KDM2A. Nucleation of KDM2A at these elements results in removal of H3K36 methylation, creating CpG island chromatin that is uniquely depleted of this modification. KDM2A utilizes a zinc finger CxxC (ZF-CxxC) domain that preferentially recognizes nonmethylated CpG DNA, and binding is blocked when the CpG DNA is methylated, thus constraining KDM2A to nonmethylated CpG islands. These data expose a straightforward mechanism through which KDM2A delineates a unique architecture that differentiates CpG island chromatin from bulk chromatin. |
| spellingShingle | Blackledge, N Zhou, J Tolstorukov, M Farcas, A Park, P Klose, R CpG islands recruit a histone H3 lysine 36 demethylase. |
| title | CpG islands recruit a histone H3 lysine 36 demethylase. |
| title_full | CpG islands recruit a histone H3 lysine 36 demethylase. |
| title_fullStr | CpG islands recruit a histone H3 lysine 36 demethylase. |
| title_full_unstemmed | CpG islands recruit a histone H3 lysine 36 demethylase. |
| title_short | CpG islands recruit a histone H3 lysine 36 demethylase. |
| title_sort | cpg islands recruit a histone h3 lysine 36 demethylase |
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