Quantitation of paracetamol by liquid chromatography-mass spectrometry in human plasma in support of clinical trial
<strong>Background:</strong> Paracetamol is a well-tolerated antipyretic widely used in severe malaria management. The study aimed to develop and validate a rapid liquid chromatography mass spectrometry (LC-MS/MS) assay to quantify paracetamol in plasma from patients with severe malaria....
| Main Authors: | , , , , , , |
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| Format: | Journal article |
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Future Science
2018
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| _version_ | 1826303272955150336 |
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| author | Plewes, K Tarning, JO Dondorp, AM Ghose, A Wong, HT Kam, RK Chan, MH |
| author_facet | Plewes, K Tarning, JO Dondorp, AM Ghose, A Wong, HT Kam, RK Chan, MH |
| author_sort | Plewes, K |
| collection | OXFORD |
| description | <strong>Background:</strong> Paracetamol is a well-tolerated antipyretic widely used in severe malaria management. The study aimed to develop and validate a rapid liquid chromatography mass spectrometry (LC-MS/MS) assay to quantify paracetamol in plasma from patients with severe malaria. <strong>Results:</strong> Plasma samples were extracted with organic solvent and assayed in less than 5.5 minutes. The assay response was linear (0.125-50 mg/L) with total intra- and inter-assay imprecision of <1.4%, which were considerably lower than most published reports. <strong>Conclusions:</strong> We developed, validated and applied a rapid, and small volume LC-MS/MS assay with high precision and accuracy for plasma paracetamol quantitation in 989 samples from 62 patients with severe malaria. The simple and high-throughput quality could facilitate assay automation for future clinical studies. |
| first_indexed | 2024-03-07T06:00:09Z |
| format | Journal article |
| id | oxford-uuid:ebe4fffc-2ad2-47f4-bde7-d4cec214d5f1 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T06:00:09Z |
| publishDate | 2018 |
| publisher | Future Science |
| record_format | dspace |
| spelling | oxford-uuid:ebe4fffc-2ad2-47f4-bde7-d4cec214d5f12022-03-27T11:13:24ZQuantitation of paracetamol by liquid chromatography-mass spectrometry in human plasma in support of clinical trialJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ebe4fffc-2ad2-47f4-bde7-d4cec214d5f1Symplectic Elements at OxfordFuture Science2018Plewes, KTarning, JODondorp, AMGhose, AWong, HTKam, RKChan, MH<strong>Background:</strong> Paracetamol is a well-tolerated antipyretic widely used in severe malaria management. The study aimed to develop and validate a rapid liquid chromatography mass spectrometry (LC-MS/MS) assay to quantify paracetamol in plasma from patients with severe malaria. <strong>Results:</strong> Plasma samples were extracted with organic solvent and assayed in less than 5.5 minutes. The assay response was linear (0.125-50 mg/L) with total intra- and inter-assay imprecision of <1.4%, which were considerably lower than most published reports. <strong>Conclusions:</strong> We developed, validated and applied a rapid, and small volume LC-MS/MS assay with high precision and accuracy for plasma paracetamol quantitation in 989 samples from 62 patients with severe malaria. The simple and high-throughput quality could facilitate assay automation for future clinical studies. |
| spellingShingle | Plewes, K Tarning, JO Dondorp, AM Ghose, A Wong, HT Kam, RK Chan, MH Quantitation of paracetamol by liquid chromatography-mass spectrometry in human plasma in support of clinical trial |
| title | Quantitation of paracetamol by liquid chromatography-mass spectrometry in human plasma in support of clinical trial |
| title_full | Quantitation of paracetamol by liquid chromatography-mass spectrometry in human plasma in support of clinical trial |
| title_fullStr | Quantitation of paracetamol by liquid chromatography-mass spectrometry in human plasma in support of clinical trial |
| title_full_unstemmed | Quantitation of paracetamol by liquid chromatography-mass spectrometry in human plasma in support of clinical trial |
| title_short | Quantitation of paracetamol by liquid chromatography-mass spectrometry in human plasma in support of clinical trial |
| title_sort | quantitation of paracetamol by liquid chromatography mass spectrometry in human plasma in support of clinical trial |
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