The effect of cysteine mutations on recombinant deacetoxycephalosporin C synthase from S. clavuligerus.
Cysteines 100, 155, and 197 of recombinant deacetoxycephalosporin C synthase were mutated to alanine residues. The C100A mutant had properties similar to those of the wild-type enzyme, but mutation of Cys-155 and Cys-197 reduced enzyme activity with penicillin N and penicillin G to different extents...
| Main Authors: | , , , |
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| 格式: | Journal article |
| 语言: | English |
| 出版: |
2000
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