The effect of cysteine mutations on recombinant deacetoxycephalosporin C synthase from S. clavuligerus.

The effect of cysteine mutations on recombinant deacetoxycephalosporin C synthase from S. clavuligerus.

Cysteines 100, 155, and 197 of recombinant deacetoxycephalosporin C synthase were mutated to alanine residues. The C100A mutant had properties similar to those of the wild-type enzyme, but mutation of Cys-155 and Cys-197 reduced enzyme activity with penicillin N and penicillin G to different extents...

Bibliografske podrobnosti
Main Authors:	Lee, H, Lloyd, MD, Harlos, K, Schofield, C
Format:	Journal article
Jezik:	English
Izdano:	2000

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