"Split-and-Click" sgRNA
CRISPR-Cas9 gene editing is dependent on a programmable single guide RNA (sgRNA) that directs Cas9 endonuclease activity. This RNA is often generated by enzymatic reactions, however the process becomes time-consuming as the number of sgRNAs increases and does not allow the incorporation of chemical...
প্রধান লেখক: | , , , |
---|---|
বিন্যাস: | Journal article |
ভাষা: | English |
প্রকাশিত: |
Springer Nature
2020
|