MHC class I gene organization in > 1.5-Mb YAC contigs from the H2-M region.
Sixteen yeast artificial chromosome (YAC) clones have been mapped to the H2-M region at the distal end of the mouse major histocompatibility complex (MHC) on chromosome 17. Analysis of the YACs with single- and multicopy probes yielded a proximal contig spanning a minimum of 800 kb and a distal cont...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1995
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| _version_ | 1826303479967121408 |
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| author | Jones, E Xiao, H Schultz, R Flaherty, L Trachtulec, Z Vincek, V Larin, Z Lehrach, H Lindahl, K |
| author_facet | Jones, E Xiao, H Schultz, R Flaherty, L Trachtulec, Z Vincek, V Larin, Z Lehrach, H Lindahl, K |
| author_sort | Jones, E |
| collection | OXFORD |
| description | Sixteen yeast artificial chromosome (YAC) clones have been mapped to the H2-M region at the distal end of the mouse major histocompatibility complex (MHC) on chromosome 17. Analysis of the YACs with single- and multicopy probes yielded a proximal contig spanning a minimum of 800 kb and a distal contig of 700 kb. A probe for the conserved fourth exon of MHC class I genes detected 19 restriction fragments, including 6 of the 8 previously characterized H2-M class I genes, in the proximal contig. This contig spans the gap from the M to the T region and includes the T1 gene. By contrast, only two class I genes, M2 and M3, were found in the distal contig. These two genes, which are both expressed, may mark the end of the MHC. The order among nine class I genes and seven other markers was determined in the cloned DNA from the centromere as T1, Tu32A, (M1-M7-M8), Tu32B, B30, M6, M4, M5, Mog, Tu42A parallel M2, Leh525, M3, Tu42B, where the orientation with respect to the centromere is unknown for M1-M7-M8. |
| first_indexed | 2024-03-07T06:03:20Z |
| format | Journal article |
| id | oxford-uuid:ecf846a3-c0f1-4a33-825b-dd0404307bd6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:03:20Z |
| publishDate | 1995 |
| record_format | dspace |
| spelling | oxford-uuid:ecf846a3-c0f1-4a33-825b-dd0404307bd62022-03-27T11:21:34ZMHC class I gene organization in > 1.5-Mb YAC contigs from the H2-M region.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ecf846a3-c0f1-4a33-825b-dd0404307bd6EnglishSymplectic Elements at Oxford1995Jones, EXiao, HSchultz, RFlaherty, LTrachtulec, ZVincek, VLarin, ZLehrach, HLindahl, KSixteen yeast artificial chromosome (YAC) clones have been mapped to the H2-M region at the distal end of the mouse major histocompatibility complex (MHC) on chromosome 17. Analysis of the YACs with single- and multicopy probes yielded a proximal contig spanning a minimum of 800 kb and a distal contig of 700 kb. A probe for the conserved fourth exon of MHC class I genes detected 19 restriction fragments, including 6 of the 8 previously characterized H2-M class I genes, in the proximal contig. This contig spans the gap from the M to the T region and includes the T1 gene. By contrast, only two class I genes, M2 and M3, were found in the distal contig. These two genes, which are both expressed, may mark the end of the MHC. The order among nine class I genes and seven other markers was determined in the cloned DNA from the centromere as T1, Tu32A, (M1-M7-M8), Tu32B, B30, M6, M4, M5, Mog, Tu42A parallel M2, Leh525, M3, Tu42B, where the orientation with respect to the centromere is unknown for M1-M7-M8. |
| spellingShingle | Jones, E Xiao, H Schultz, R Flaherty, L Trachtulec, Z Vincek, V Larin, Z Lehrach, H Lindahl, K MHC class I gene organization in > 1.5-Mb YAC contigs from the H2-M region. |
| title | MHC class I gene organization in > 1.5-Mb YAC contigs from the H2-M region. |
| title_full | MHC class I gene organization in > 1.5-Mb YAC contigs from the H2-M region. |
| title_fullStr | MHC class I gene organization in > 1.5-Mb YAC contigs from the H2-M region. |
| title_full_unstemmed | MHC class I gene organization in > 1.5-Mb YAC contigs from the H2-M region. |
| title_short | MHC class I gene organization in > 1.5-Mb YAC contigs from the H2-M region. |
| title_sort | mhc class i gene organization in gt 1 5 mb yac contigs from the h2 m region |
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