Nature of T cell-macrophage interaction in helper cell induction in vitro. I. Evidence for genetic restriction of T cell-macrophage interactions prior to T cell priming.
The nature of the genetic restriction of T cell-macrophage interaction in helper cell induction has been investigated. By incubating F 1 hybrid T cells with keyhole limpet hemocyanin and macrophages of one parental haplotype, helper cells were generated that cooperated with B cells of either parenta...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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1978
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| _version_ | 1797102262578839552 |
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| author | Erb, P Meier, B Kraus, D von Boehmer, H Feldmann, M |
| author_facet | Erb, P Meier, B Kraus, D von Boehmer, H Feldmann, M |
| author_sort | Erb, P |
| collection | OXFORD |
| description | The nature of the genetic restriction of T cell-macrophage interaction in helper cell induction has been investigated. By incubating F 1 hybrid T cells with keyhole limpet hemocyanin and macrophages of one parental haplotype, helper cells were generated that cooperated with B cells of either parental haplotype, provided macrophages of the haplotype used for the initial priming were present in the cooperative culture. The experiment is in line with the view that F 1 T cells reactive to a given antigen contain at least two independent clones, each responsive to antigen recognized in conjunction with macrophages of one parental strain, and moreover, indicates that genetic restriction of T cell-macrophage interaction may be induced by priming. However, by using F 1 T cells it is not possible to exclude that restriction is already manifest prior to priming. Thus, experiments were performed using T cells from chimeric mice instead of F 1 mice. Chimeric mice, prepared by injecting lethally irradiated F 1 mice with anti-Θ-treated bone marrow cells of both parental types, have two histoincompatible lymphoid pools which are mutually tolerant. T cells of one type, prepared by treatment with anti H-2 serum and complement, were not able to interact with macrophages of the other histoincompatible parental type indicating that genetic restrictions in interaction may be expressed prior to antigenic stimulation. The possibility that suppression is the mechanism of the restriction of T cell-macrophage interaction in helper cell induction has been investigated and excluded, based on several lines of evidence. First, genetic restriction is not broken by treatment of helper cells induced by allogeneic genetically related macrophage factors (GRF) (or mixtures of syngeneic and allogeneic GFR) with antisera which kill suppressor cells, which may have masked the help induced with allogeneic GFR. Second, chimeric mice, which have not been shown to have suppressor cells, still show the same genetic restriction. The implication of these findings for patterns of T cell recognition is discussed. |
| first_indexed | 2024-03-07T06:03:27Z |
| format | Journal article |
| id | oxford-uuid:ed05b544-e951-4c7b-8858-2287b46282f8 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:03:27Z |
| publishDate | 1978 |
| record_format | dspace |
| spelling | oxford-uuid:ed05b544-e951-4c7b-8858-2287b46282f82022-03-27T11:21:48ZNature of T cell-macrophage interaction in helper cell induction in vitro. I. Evidence for genetic restriction of T cell-macrophage interactions prior to T cell priming.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ed05b544-e951-4c7b-8858-2287b46282f8EnglishSymplectic Elements at Oxford1978Erb, PMeier, BKraus, Dvon Boehmer, HFeldmann, MThe nature of the genetic restriction of T cell-macrophage interaction in helper cell induction has been investigated. By incubating F 1 hybrid T cells with keyhole limpet hemocyanin and macrophages of one parental haplotype, helper cells were generated that cooperated with B cells of either parental haplotype, provided macrophages of the haplotype used for the initial priming were present in the cooperative culture. The experiment is in line with the view that F 1 T cells reactive to a given antigen contain at least two independent clones, each responsive to antigen recognized in conjunction with macrophages of one parental strain, and moreover, indicates that genetic restriction of T cell-macrophage interaction may be induced by priming. However, by using F 1 T cells it is not possible to exclude that restriction is already manifest prior to priming. Thus, experiments were performed using T cells from chimeric mice instead of F 1 mice. Chimeric mice, prepared by injecting lethally irradiated F 1 mice with anti-Θ-treated bone marrow cells of both parental types, have two histoincompatible lymphoid pools which are mutually tolerant. T cells of one type, prepared by treatment with anti H-2 serum and complement, were not able to interact with macrophages of the other histoincompatible parental type indicating that genetic restrictions in interaction may be expressed prior to antigenic stimulation. The possibility that suppression is the mechanism of the restriction of T cell-macrophage interaction in helper cell induction has been investigated and excluded, based on several lines of evidence. First, genetic restriction is not broken by treatment of helper cells induced by allogeneic genetically related macrophage factors (GRF) (or mixtures of syngeneic and allogeneic GFR) with antisera which kill suppressor cells, which may have masked the help induced with allogeneic GFR. Second, chimeric mice, which have not been shown to have suppressor cells, still show the same genetic restriction. The implication of these findings for patterns of T cell recognition is discussed. |
| spellingShingle | Erb, P Meier, B Kraus, D von Boehmer, H Feldmann, M Nature of T cell-macrophage interaction in helper cell induction in vitro. I. Evidence for genetic restriction of T cell-macrophage interactions prior to T cell priming. |
| title | Nature of T cell-macrophage interaction in helper cell induction in vitro. I. Evidence for genetic restriction of T cell-macrophage interactions prior to T cell priming. |
| title_full | Nature of T cell-macrophage interaction in helper cell induction in vitro. I. Evidence for genetic restriction of T cell-macrophage interactions prior to T cell priming. |
| title_fullStr | Nature of T cell-macrophage interaction in helper cell induction in vitro. I. Evidence for genetic restriction of T cell-macrophage interactions prior to T cell priming. |
| title_full_unstemmed | Nature of T cell-macrophage interaction in helper cell induction in vitro. I. Evidence for genetic restriction of T cell-macrophage interactions prior to T cell priming. |
| title_short | Nature of T cell-macrophage interaction in helper cell induction in vitro. I. Evidence for genetic restriction of T cell-macrophage interactions prior to T cell priming. |
| title_sort | nature of t cell macrophage interaction in helper cell induction in vitro i evidence for genetic restriction of t cell macrophage interactions prior to t cell priming |
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