Triazine probes targeting ascorbate peroxidases in plants
Though they are rare in nature, anthropogenic 1,3,5-triazines have been used in herbicides as chemically stable scaffolds. Here, we show that small 1,3,5-triazines selectively target ascorbate peroxidases (APXs) in Arabidopsis (Arabidopsis thaliana), tomato (Solanum lycopersicum), rice (Oryza sativa...
| প্রধান লেখক: | , , , , , , , , , , , , , |
|---|---|
| বিন্যাস: | Journal article |
| ভাষা: | English |
| প্রকাশিত: |
American Society of Plant Biologists
2019
|
| _version_ | 1826303511826006016 |
|---|---|
| author | Morimoto, K Cole, K Kourelis, J Witt, C Brown, D Krahn, D Stegmann, M Kaschani, F Kaiser, M Burton, J Mohammed, S Yamaguchi-Shinozaki, K Weerapana, E Van Der Hoorn, R |
| author_facet | Morimoto, K Cole, K Kourelis, J Witt, C Brown, D Krahn, D Stegmann, M Kaschani, F Kaiser, M Burton, J Mohammed, S Yamaguchi-Shinozaki, K Weerapana, E Van Der Hoorn, R |
| author_sort | Morimoto, K |
| collection | OXFORD |
| description | Though they are rare in nature, anthropogenic 1,3,5-triazines have been used in herbicides as chemically stable scaffolds. Here, we show that small 1,3,5-triazines selectively target ascorbate peroxidases (APXs) in Arabidopsis (Arabidopsis thaliana), tomato (Solanum lycopersicum), rice (Oryza sativa), maize (Zea mays), liverwort (Marchantia polymorpha), and other plant species. The alkyne-tagged 2-chloro-4-methyl-1,3,5-triazine probe KSC-3 selectively binds APX enzymes, both in crude extracts and in living cells. KSC-3 blocks APX activity, thereby reducing photosynthetic activity under moderate light stress, even in apx1 mutant plants. This suggests that APX enzymes in addition to APX1 protect the photosystem against reactive oxygen species. Profiling APX1 with KCS-3 revealed that the catabolic products of atrazine (a 1,3,5-triazine herbicide), which are common soil pollutants, also target APX1. Thus, KSC-3 is a powerful chemical probe to study APX enzymes in the plant kingdom. |
| first_indexed | 2024-03-07T06:03:49Z |
| format | Journal article |
| id | oxford-uuid:ed22c3db-7b4b-4b9c-9d4c-4ba3a67a8a71 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:03:49Z |
| publishDate | 2019 |
| publisher | American Society of Plant Biologists |
| record_format | dspace |
| spelling | oxford-uuid:ed22c3db-7b4b-4b9c-9d4c-4ba3a67a8a712022-03-27T11:22:48ZTriazine probes targeting ascorbate peroxidases in plantsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ed22c3db-7b4b-4b9c-9d4c-4ba3a67a8a71EnglishSymplectic Elements at OxfordAmerican Society of Plant Biologists2019Morimoto, KCole, KKourelis, JWitt, CBrown, DKrahn, DStegmann, MKaschani, FKaiser, MBurton, JMohammed, SYamaguchi-Shinozaki, KWeerapana, EVan Der Hoorn, RThough they are rare in nature, anthropogenic 1,3,5-triazines have been used in herbicides as chemically stable scaffolds. Here, we show that small 1,3,5-triazines selectively target ascorbate peroxidases (APXs) in Arabidopsis (Arabidopsis thaliana), tomato (Solanum lycopersicum), rice (Oryza sativa), maize (Zea mays), liverwort (Marchantia polymorpha), and other plant species. The alkyne-tagged 2-chloro-4-methyl-1,3,5-triazine probe KSC-3 selectively binds APX enzymes, both in crude extracts and in living cells. KSC-3 blocks APX activity, thereby reducing photosynthetic activity under moderate light stress, even in apx1 mutant plants. This suggests that APX enzymes in addition to APX1 protect the photosystem against reactive oxygen species. Profiling APX1 with KCS-3 revealed that the catabolic products of atrazine (a 1,3,5-triazine herbicide), which are common soil pollutants, also target APX1. Thus, KSC-3 is a powerful chemical probe to study APX enzymes in the plant kingdom. |
| spellingShingle | Morimoto, K Cole, K Kourelis, J Witt, C Brown, D Krahn, D Stegmann, M Kaschani, F Kaiser, M Burton, J Mohammed, S Yamaguchi-Shinozaki, K Weerapana, E Van Der Hoorn, R Triazine probes targeting ascorbate peroxidases in plants |
| title | Triazine probes targeting ascorbate peroxidases in plants |
| title_full | Triazine probes targeting ascorbate peroxidases in plants |
| title_fullStr | Triazine probes targeting ascorbate peroxidases in plants |
| title_full_unstemmed | Triazine probes targeting ascorbate peroxidases in plants |
| title_short | Triazine probes targeting ascorbate peroxidases in plants |
| title_sort | triazine probes targeting ascorbate peroxidases in plants |
| work_keys_str_mv | AT morimotok triazineprobestargetingascorbateperoxidasesinplants AT colek triazineprobestargetingascorbateperoxidasesinplants AT kourelisj triazineprobestargetingascorbateperoxidasesinplants AT wittc triazineprobestargetingascorbateperoxidasesinplants AT brownd triazineprobestargetingascorbateperoxidasesinplants AT krahnd triazineprobestargetingascorbateperoxidasesinplants AT stegmannm triazineprobestargetingascorbateperoxidasesinplants AT kaschanif triazineprobestargetingascorbateperoxidasesinplants AT kaiserm triazineprobestargetingascorbateperoxidasesinplants AT burtonj triazineprobestargetingascorbateperoxidasesinplants AT mohammeds triazineprobestargetingascorbateperoxidasesinplants AT yamaguchishinozakik triazineprobestargetingascorbateperoxidasesinplants AT weerapanae triazineprobestargetingascorbateperoxidasesinplants AT vanderhoornr triazineprobestargetingascorbateperoxidasesinplants |