Regulation of proteoglycan synthesis rate in cartilage in vitro: influence of extracellular ionic composition.
Load-bearing cartilages regularly experience changes in fluid content as the result of changing load. It has been found that these changes in fluid content influence proteoglycan synthesis. The mechanism for this effect is not known. We have measured the influence of changes in cartilage hydration o...
| Autores principales: | , |
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| Formato: | Journal article |
| Lenguaje: | English |
| Publicado: |
1989
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| _version_ | 1826303558129025024 |
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| author | Urban, J Bayliss, M |
| author_facet | Urban, J Bayliss, M |
| author_sort | Urban, J |
| collection | OXFORD |
| description | Load-bearing cartilages regularly experience changes in fluid content as the result of changing load. It has been found that these changes in fluid content influence proteoglycan synthesis. The mechanism for this effect is not known. We have measured the influence of changes in cartilage hydration on the [35S]sulphate incorporation rate in both bovine nasal and human articular cartilage in medium whose concentration varied over the range 0.2-2-times physiological strength. In physiological medium the incorporation rate fell in proportion to fluid loss with a 10% fall in cartilage hydration resulting in a 30-50% decrease in 35S-incorporation rates. However, in medium of 0.5-times physiological strength, where the incorporation rate was only 40% of control values, the incorporation rate increased initially rather than falling as the cartilage lost fluid. These changes in hydration and hence proteoglycan content resulted in changes in the extracellular ionic composition of cartilage. When this was monitored in terms of [Na+]c, the internal sodium concentration, as a marker for changes in cartilage ionic composition, we found that incorporation rate varied with [Na+]c rather than directly with hydration. |
| first_indexed | 2024-03-07T06:04:31Z |
| format | Journal article |
| id | oxford-uuid:ed5e7661-2d65-4625-b6c8-a5e740b4931a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:04:31Z |
| publishDate | 1989 |
| record_format | dspace |
| spelling | oxford-uuid:ed5e7661-2d65-4625-b6c8-a5e740b4931a2022-03-27T11:24:28ZRegulation of proteoglycan synthesis rate in cartilage in vitro: influence of extracellular ionic composition.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ed5e7661-2d65-4625-b6c8-a5e740b4931aEnglishSymplectic Elements at Oxford1989Urban, JBayliss, MLoad-bearing cartilages regularly experience changes in fluid content as the result of changing load. It has been found that these changes in fluid content influence proteoglycan synthesis. The mechanism for this effect is not known. We have measured the influence of changes in cartilage hydration on the [35S]sulphate incorporation rate in both bovine nasal and human articular cartilage in medium whose concentration varied over the range 0.2-2-times physiological strength. In physiological medium the incorporation rate fell in proportion to fluid loss with a 10% fall in cartilage hydration resulting in a 30-50% decrease in 35S-incorporation rates. However, in medium of 0.5-times physiological strength, where the incorporation rate was only 40% of control values, the incorporation rate increased initially rather than falling as the cartilage lost fluid. These changes in hydration and hence proteoglycan content resulted in changes in the extracellular ionic composition of cartilage. When this was monitored in terms of [Na+]c, the internal sodium concentration, as a marker for changes in cartilage ionic composition, we found that incorporation rate varied with [Na+]c rather than directly with hydration. |
| spellingShingle | Urban, J Bayliss, M Regulation of proteoglycan synthesis rate in cartilage in vitro: influence of extracellular ionic composition. |
| title | Regulation of proteoglycan synthesis rate in cartilage in vitro: influence of extracellular ionic composition. |
| title_full | Regulation of proteoglycan synthesis rate in cartilage in vitro: influence of extracellular ionic composition. |
| title_fullStr | Regulation of proteoglycan synthesis rate in cartilage in vitro: influence of extracellular ionic composition. |
| title_full_unstemmed | Regulation of proteoglycan synthesis rate in cartilage in vitro: influence of extracellular ionic composition. |
| title_short | Regulation of proteoglycan synthesis rate in cartilage in vitro: influence of extracellular ionic composition. |
| title_sort | regulation of proteoglycan synthesis rate in cartilage in vitro influence of extracellular ionic composition |
| work_keys_str_mv | AT urbanj regulationofproteoglycansynthesisrateincartilageinvitroinfluenceofextracellularioniccomposition AT baylissm regulationofproteoglycansynthesisrateincartilageinvitroinfluenceofextracellularioniccomposition |