Breaking the diffraction barrier in fluorescence microscopy by optical shelving.
We report the breaking of the diffraction resolution barrier in far-field fluorescence microscopy by transiently shelving the fluorophore in a metastable dark state. Using a relatively modest light intensity of several kW/cm(2) in a focal distribution featuring a local zero, we confine the fluoresce...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2007
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Breaking the diffraction barrier in fluorescence microscopy by optical shelving
Published 2007
Journal article