Design and validation of an enzyme-linked immunospot assay for use in clinical trials of candidate HIV vaccines.

Design and validation of an enzyme-linked immunospot assay for use in clinical trials of candidate HIV vaccines.

The enzyme-linked immunosorbent (ELISPOT) assay, which enumerates peripheral blood mononuclear cells (PBMCs) releasing interferon gamma (IFN-gamma) on specific antigen stimulation, is becoming the assay of choice for evaluation of vaccine-induced cell-mediated immune responses in many clinical trial...

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Bibliographic Details
Main Authors:	Mwau, M, Mcmichael, A, Hanke, T
Format:	Journal article
Language:	English
Published:	2002

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