Design and validation of an enzyme-linked immunospot assay for use in clinical trials of candidate HIV vaccines.

Design and validation of an enzyme-linked immunospot assay for use in clinical trials of candidate HIV vaccines.

The enzyme-linked immunosorbent (ELISPOT) assay, which enumerates peripheral blood mononuclear cells (PBMCs) releasing interferon gamma (IFN-gamma) on specific antigen stimulation, is becoming the assay of choice for evaluation of vaccine-induced cell-mediated immune responses in many clinical trial...

Descrición completa

Detalles Bibliográficos
Main Authors:	Mwau, M, Mcmichael, A, Hanke, T
Formato:	Journal article
Idioma:	English
Publicado:	2002

Títulos similares

Diagnosis of Tuberculosis by an Enzyme-Linked Immunospot Assay for Interferon-γ
por: Jann-Yuan Wang, et al.
Publicado: (2007-04-01)

Enumeration and Characterization of Human Memory T Cells by Enzyme-Linked Immunospot Assays
por: Sandra A. Calarota, et al.
Publicado: (2013-01-01)

Cost-Efficient Quantification of Enzyme-Linked Immunospot
por: Mandvi Bharadwaj, et al.
Publicado: (2001-01-01)

Digital Quantification of the Enzyme-Linked Immunospot (ELISPOT)
por: J.E. Vaquerano, et al.
Publicado: (1998-11-01)

Design and tests of an HIV vaccine.
por: McMichael, A, et al.
Publicado: (2002)

Use of enzyme linked immunospot assay (ELISpot) for monitoring treatment response of pulmonary tuberculosis patients
por: Ramadan M. Nafae, et al.
Publicado: (2013-07-01)

Bronchoalveolar lavage enzyme-linked immunospot for diagnosis of smear-negative tuberculosis in HIV-infected patients.
por: Adithya Cattamanchi, et al.
Publicado: (2012-01-01)

Cultured enzyme-linked immunospot assay (ELISPOT) enhances detection of low HIV-specific immune responses in exposed seronegative individuals in Kenya
por: Anzala Omu, et al.
Publicado: (2006-12-01)

Development of a rapid neutralization testing system for Rhinovirus C15 based on the enzyme-linked immunospot assay
por: Zhenhong Zhou, et al.
Publicado: (2022-09-01)

HIV T cell vaccines, the importance of clades.
por: McMichael, A, et al.
Publicado: (2002)

Evaluation of an enzyme-linked immunospot assay for the immunodiagnosis of atypical spinal tuberculosis (atypical clinical presentation/atypical radiographic presentation) in China
por: Kai Yuan, et al.
Publicado: (2013-09-01)

Development and evaluation of a Mycobacterium bovis interferon-γ enzyme-linked immunospot (ELISpot) assay for detection of bovine tuberculosis
por: Xin Li, et al.
Publicado: (2022-07-01)

Concordant proficiency in measurement of T-cell immunity in human immunodeficiency virus vaccine clinical trials by peripheral blood mononuclear cell and enzyme-linked immunospot assays in laboratories from three continents.
por: Boaz, M, et al.
Publicado: (2009)

Complete mapping of a novel HLA A*6801-restricted HIV-1 Tat epitope directly with a rapid modified enzyme-linked immunospot assay.
por: Oxenius, A, et al.
Publicado: (2002)

Characterization of Varicella-Zoster (VZV) Specific T Cell Response in Healthy Subjects and Transplanted Patients by Using Enzyme Linked Immunospot (ELISpot) Assays
por: Irene Cassaniti, et al.
Publicado: (2021-08-01)

Usefulness of BK virus-specific interferon-γ enzyme-linked immunospot assay for predicting the outcome of BK virus infection in kidney transplant recipients
por: Hyunjoo Bae, et al.
Publicado: (2021-01-01)

Computer-Assisted, Quantitative Cytokine Enzyme-Linked Immunospot Analysis of Human Immune Effector Cell Function
por: Yan Cui, et al.
Publicado: (1997-06-01)

Design and construction of an experimental HIV-1 vaccine for a year-2000 clinical trial in Kenya.
por: Hanke, T, et al.
Publicado: (2000)

Durable human memory T cells quantifiable by cultured enzyme-linked immunospot assays are induced by heterologous prime boost immunization and correlate with protection against malaria.
por: Keating, S, et al.
Publicado: (2005)

A modified anthrax toxin-based enzyme-linked immunospot assay reveals robust T cell responses in symptomatic and asymptomatic Ebola virus exposed individuals.
por: Bobby Brooke Herrera, et al.
Publicado: (2018-05-01)

Evaluation of EBV- and HCMV-Specific T Cell Responses in Systemic Lupus Erythematosus (SLE) Patients Using a Normalized Enzyme-Linked Immunospot (ELISPOT) Assay
por: Irene Cassaniti, et al.
Publicado: (2019-01-01)

Harmonization and qualification of an IFN-γ Enzyme-Linked ImmunoSpot assay (ELISPOT) to measure influenza-specific cell-mediated immunity within the FLUCOP consortium
por: Gwenn Waerlop, et al.
Publicado: (2022-09-01)

Comparison between enzyme‐linked immunospot assay and intracellular cytokine flow cytometry assays for the evaluation of T cell response to SARS‐CoV‐2 after symptomatic COVID‐19
por: Juliette Villemonteix, et al.
Publicado: (2022-10-01)

A review of vaccines for HIV prevention.
por: Mwau, M, et al.
Publicado: (2003)

Identification of dominant optimal HLA-B60- and HLA-B61-restricted cytotoxic T-lymphocyte (CTL) epitopes: rapid characterization of CTL responses by enzyme-linked immunospot assay.
por: Altfeld, M, et al.
Publicado: (2000)

Chronic-Relapsing cutaneous leukocytoclastic vasculitis in a young patient with reduced EBV-specific T cell response using enzyme-linked immunospot (ELISPOT) assay successfully treated with Valaciclovir
por: Caterina Foti, et al.
Publicado: (2021-01-01)

Affinity Tag Coating Enables Reliable Detection of Antigen-Specific B Cells in Immunospot Assays
por: Sebastian Köppert, et al.
Publicado: (2021-07-01)

Is an HIV vaccine possible?
por: Mcmichael, A, et al.
Publicado: (1999)

The Importance of Monitoring Antigen-Specific Memory B Cells, and How ImmunoSpot Assays Are Suitable for This Task
por: Greg A. Kirchenbaum, et al.
Publicado: (2025-02-01)

Validation of the Enzyme-Linked ImmunoSpot Analytic Method for the Detection of Human IFN-γ from Peripheral Blood Mononuclear Cells in Response to the SARS-CoV-2 Spike Protein
por: Laura E. Carreto-Binaghi, et al.
Publicado: (2024-10-01)

HIV vaccines 1983-2003.
por: Mcmichael, A, et al.
Publicado: (2003)

Comment on clinical development of candidate HIV vaccines: different problems for different vaccines.
por: McMichael, A, et al.
Publicado: (2014)

Development and Validation of Enzyme-Linked Immunosorbent Assay for Group B Streptococcal Polysaccharide Vaccine
por: A-Yeung Jang, et al.
Publicado: (2021-05-01)

Cardiac Safety in Clinical Trials with Preventive HIV Vaccine Candidates Based on Modified Vaccinia Ankara (MVA)
por: Schmidt, C, et al.
Publicado: (2008)

Novel HIV-1 clade B candidate vaccines designed for HLA-B*5101(+) patients protected mice against chimaeric ecotropic HIV-1 challenge.
por: Roshorm, Y, et al.
Publicado: (2009)

Biodistribution and persistence of an MVA-vectored candidate HIV vaccine in SIV-infected rhesus macaques and SCID mice.
por: Hanke, T, et al.
Publicado: (2005)

Development and Validation of an Enzyme-Linked Immunosorbent Assay-Based Protocol for Evaluation of Respiratory Syncytial Virus Vaccines
por: Eliel Nham, et al.
Publicado: (2024-06-01)

Selection of Vaccine Candidate for Foot-and-Mouth Disease Virus Serotype O Using a Blocking Enzyme-Linked Immunosorbent Assay
por: Yimei Cao, et al.
Publicado: (2021-04-01)

Combined single-clade candidate HIV-1 vaccines induce T cell responses limited by multiple forms of in vivo immune interference.
por: Larke, N, et al.
Publicado: (2007)

Enumeration of functional T-cell subsets by fluorescence-immunospot defines signatures of pathogen burden in tuberculosis.
por: Rosalyn Casey, et al.
Publicado: (2010-12-01)