Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2'-deoxyuridine.
We report procedures to allow incorporation and detection of 5-ethynyl-2'-deoxyuridine (EdU) in fission yeast, a thymidine analogue which has some technical advantages over use of bromodeoxyuridine. Low concentrations of EdU (1 µM) are sufficient to allow detection of incorporation in cells exp...
| Main Authors: | , |
|---|---|
| 格式: | Journal article |
| 語言: | English |
| 出版: |
2011
|