The effects of COST on the differentiation of 3T3-L1 preadipocytes and the mechanism of action.
The objectives of this study were to explore the effect of COST (one thousand Da molecular weight chitosan oligosaccharide) on the differentiation of 3T3-L1 preadipocytes and to determine the mechanism of action. 3T3-L1 preadipocytes were used as the target cells, and the induction of the methods fo...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Elsevier
2016
|
| _version_ | 1797102387254525952 |
|---|---|
| author | Kong, S Ding, C Huang, L Bai, Y Xiao, T Guo, J Su, Z |
| author_facet | Kong, S Ding, C Huang, L Bai, Y Xiao, T Guo, J Su, Z |
| author_sort | Kong, S |
| collection | OXFORD |
| description | The objectives of this study were to explore the effect of COST (one thousand Da molecular weight chitosan oligosaccharide) on the differentiation of 3T3-L1 preadipocytes and to determine the mechanism of action. 3T3-L1 preadipocytes were used as the target cells, and the induction of the methods for the differentiation of 3T3-L1 preadipocytes was based on classic cocktails. The MTT assay was used to filtrate the concentration of COST. On the 6th day of induced-differentiation, the differentiation of 3T3-L1 cells was detected by Oil Red O staining. The expression of PPARγ and C/EBPα mRNA was determined using real-time fluorescence quantitative PCR (Q-PCR). COST inhibited 3T3-L1 preadipocyte differentiation in a dose-dependent manner and decreased lipid accumulation. At the molecular level, the expression of the transcription factors, PPARγ and C/EBPα, was reduced by COST during adipogenesis. These results indicate that COST effectively inhibited the differentiation of 3T3-L1 preadipocytes. The mechanism is related to the down-regulation expression of PPARγ and C/EBPα. |
| first_indexed | 2024-03-07T06:05:17Z |
| format | Journal article |
| id | oxford-uuid:eda053c3-0dff-4bb0-8b0b-0b49ff84ded6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:05:17Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:eda053c3-0dff-4bb0-8b0b-0b49ff84ded62022-03-27T11:26:31ZThe effects of COST on the differentiation of 3T3-L1 preadipocytes and the mechanism of action.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:eda053c3-0dff-4bb0-8b0b-0b49ff84ded6EnglishSymplectic Elements at OxfordElsevier2016Kong, SDing, CHuang, LBai, YXiao, TGuo, JSu, ZThe objectives of this study were to explore the effect of COST (one thousand Da molecular weight chitosan oligosaccharide) on the differentiation of 3T3-L1 preadipocytes and to determine the mechanism of action. 3T3-L1 preadipocytes were used as the target cells, and the induction of the methods for the differentiation of 3T3-L1 preadipocytes was based on classic cocktails. The MTT assay was used to filtrate the concentration of COST. On the 6th day of induced-differentiation, the differentiation of 3T3-L1 cells was detected by Oil Red O staining. The expression of PPARγ and C/EBPα mRNA was determined using real-time fluorescence quantitative PCR (Q-PCR). COST inhibited 3T3-L1 preadipocyte differentiation in a dose-dependent manner and decreased lipid accumulation. At the molecular level, the expression of the transcription factors, PPARγ and C/EBPα, was reduced by COST during adipogenesis. These results indicate that COST effectively inhibited the differentiation of 3T3-L1 preadipocytes. The mechanism is related to the down-regulation expression of PPARγ and C/EBPα. |
| spellingShingle | Kong, S Ding, C Huang, L Bai, Y Xiao, T Guo, J Su, Z The effects of COST on the differentiation of 3T3-L1 preadipocytes and the mechanism of action. |
| title | The effects of COST on the differentiation of 3T3-L1 preadipocytes and the mechanism of action. |
| title_full | The effects of COST on the differentiation of 3T3-L1 preadipocytes and the mechanism of action. |
| title_fullStr | The effects of COST on the differentiation of 3T3-L1 preadipocytes and the mechanism of action. |
| title_full_unstemmed | The effects of COST on the differentiation of 3T3-L1 preadipocytes and the mechanism of action. |
| title_short | The effects of COST on the differentiation of 3T3-L1 preadipocytes and the mechanism of action. |
| title_sort | effects of cost on the differentiation of 3t3 l1 preadipocytes and the mechanism of action |
| work_keys_str_mv | AT kongs theeffectsofcostonthedifferentiationof3t3l1preadipocytesandthemechanismofaction AT dingc theeffectsofcostonthedifferentiationof3t3l1preadipocytesandthemechanismofaction AT huangl theeffectsofcostonthedifferentiationof3t3l1preadipocytesandthemechanismofaction AT baiy theeffectsofcostonthedifferentiationof3t3l1preadipocytesandthemechanismofaction AT xiaot theeffectsofcostonthedifferentiationof3t3l1preadipocytesandthemechanismofaction AT guoj theeffectsofcostonthedifferentiationof3t3l1preadipocytesandthemechanismofaction AT suz theeffectsofcostonthedifferentiationof3t3l1preadipocytesandthemechanismofaction AT kongs effectsofcostonthedifferentiationof3t3l1preadipocytesandthemechanismofaction AT dingc effectsofcostonthedifferentiationof3t3l1preadipocytesandthemechanismofaction AT huangl effectsofcostonthedifferentiationof3t3l1preadipocytesandthemechanismofaction AT baiy effectsofcostonthedifferentiationof3t3l1preadipocytesandthemechanismofaction AT xiaot effectsofcostonthedifferentiationof3t3l1preadipocytesandthemechanismofaction AT guoj effectsofcostonthedifferentiationof3t3l1preadipocytesandthemechanismofaction AT suz effectsofcostonthedifferentiationof3t3l1preadipocytesandthemechanismofaction |