Tissue-specific histone modification and transcription factor binding in alpha globin gene expression.
To address the mechanism by which the human globin genes are activated during erythropoiesis, we have used a tiled microarray to analyze the pattern of transcription factor binding and associated histone modifications across the telomeric region of human chromosome 16 in primary erythroid and nonery...
| Main Authors: | , , , , , , , , , |
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| פורמט: | Journal article |
| שפה: | English |
| יצא לאור: |
2007
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| _version_ | 1826303634978111488 |
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| author | De Gobbi, M Anguita, E Hughes, J Sloane-Stanley, J Sharpe, J Koch, C Dunham, I Gibbons, R Wood, W Higgs, D |
| author_facet | De Gobbi, M Anguita, E Hughes, J Sloane-Stanley, J Sharpe, J Koch, C Dunham, I Gibbons, R Wood, W Higgs, D |
| author_sort | De Gobbi, M |
| collection | OXFORD |
| description | To address the mechanism by which the human globin genes are activated during erythropoiesis, we have used a tiled microarray to analyze the pattern of transcription factor binding and associated histone modifications across the telomeric region of human chromosome 16 in primary erythroid and nonerythroid cells. This 220-kb region includes the alpha globin genes and 9 widely expressed genes flanking the alpha globin locus. This un-biased, comprehensive analysis of transcription factor binding and histone modifications (acetylation and methylation) described here not only identified all known cis-acting regulatory elements in the human alpha globin cluster but also demonstrated that there are no additional erythroid-specific regulatory elements in the 220-kb region tested. In addition, the pattern of histone modification distinguished promoter elements from potential enhancer elements across this region. Finally, comparison of the human and mouse orthologous regions in a unique mouse model, with both regions coexpressed in the same animal, showed significant differences that may explain how these 2 clusters are regulated differently in vivo. |
| first_indexed | 2024-03-07T06:05:42Z |
| format | Journal article |
| id | oxford-uuid:edc2afb9-dbba-426e-a18b-679af27ab84c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:05:42Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:edc2afb9-dbba-426e-a18b-679af27ab84c2022-03-27T11:27:43ZTissue-specific histone modification and transcription factor binding in alpha globin gene expression.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:edc2afb9-dbba-426e-a18b-679af27ab84cEnglishSymplectic Elements at Oxford2007De Gobbi, MAnguita, EHughes, JSloane-Stanley, JSharpe, JKoch, CDunham, IGibbons, RWood, WHiggs, DTo address the mechanism by which the human globin genes are activated during erythropoiesis, we have used a tiled microarray to analyze the pattern of transcription factor binding and associated histone modifications across the telomeric region of human chromosome 16 in primary erythroid and nonerythroid cells. This 220-kb region includes the alpha globin genes and 9 widely expressed genes flanking the alpha globin locus. This un-biased, comprehensive analysis of transcription factor binding and histone modifications (acetylation and methylation) described here not only identified all known cis-acting regulatory elements in the human alpha globin cluster but also demonstrated that there are no additional erythroid-specific regulatory elements in the 220-kb region tested. In addition, the pattern of histone modification distinguished promoter elements from potential enhancer elements across this region. Finally, comparison of the human and mouse orthologous regions in a unique mouse model, with both regions coexpressed in the same animal, showed significant differences that may explain how these 2 clusters are regulated differently in vivo. |
| spellingShingle | De Gobbi, M Anguita, E Hughes, J Sloane-Stanley, J Sharpe, J Koch, C Dunham, I Gibbons, R Wood, W Higgs, D Tissue-specific histone modification and transcription factor binding in alpha globin gene expression. |
| title | Tissue-specific histone modification and transcription factor binding in alpha globin gene expression. |
| title_full | Tissue-specific histone modification and transcription factor binding in alpha globin gene expression. |
| title_fullStr | Tissue-specific histone modification and transcription factor binding in alpha globin gene expression. |
| title_full_unstemmed | Tissue-specific histone modification and transcription factor binding in alpha globin gene expression. |
| title_short | Tissue-specific histone modification and transcription factor binding in alpha globin gene expression. |
| title_sort | tissue specific histone modification and transcription factor binding in alpha globin gene expression |
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