The P aracoccus denitrificans NarK-like nitrate and nitrite transporters—probing nitrate uptake and nitrate/nitrite exchange mechanisms
Nitrate and nitrite transport across biological membranes is often facilitated by protein transporters that are members of the major facilitator superfamily. Paracoccus denitrificans contains an unusual arrangement whereby two of these transporters, NarK1 and NarK2, are fused into a...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
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Wiley
2016
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| _version_ | 1826303650903883776 |
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| author | Ferguson, S Goddard, A Bali, S Mavridou, D Luque-Almagro, V Gates, A Roldan, M Newstead, S Richardson, D |
| author_facet | Ferguson, S Goddard, A Bali, S Mavridou, D Luque-Almagro, V Gates, A Roldan, M Newstead, S Richardson, D |
| author_sort | Ferguson, S |
| collection | OXFORD |
| description | Nitrate and nitrite transport across biological membranes is often facilitated by protein transporters that are members of the major facilitator superfamily. Paracoccus denitrificans contains an unusual arrangement whereby two of these transporters, NarK1 and NarK2, are fused into a single protein, NarK, which delivers nitrate to the respiratory nitrate reductase and transfers the product, nitrite, to the periplasm. Our complementation studies, using a mutant lacking the nitrate/proton symporter NasA from the assimilatory nitrate reductase pathway, support that NarK1 functions as a nitrate/proton symporter while NarK2 is a nitrate/nitrite antiporter. Through the same experimental system, we find that Escherichia coli NarK and NarU can complement deletions in both narKand nasAin P. denitrificans, suggesting that, while these proteins are most likely nitrate/nitrite antiporters, they can also act in the net uptake of nitrate. Finally, we argue that primary sequence analysis and structural modelling do not readily explain why NasA, NarK1 and NarK2, as well as othertransporters from this protein family, have such different functions, ranging fromnet nitrate uptake to nitrate/nitrite exchange. |
| first_indexed | 2024-03-07T06:05:56Z |
| format | Journal article |
| id | oxford-uuid:edd7e44f-6d5a-47c6-91bd-55325377efe4 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T06:05:56Z |
| publishDate | 2016 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:edd7e44f-6d5a-47c6-91bd-55325377efe42022-03-27T11:28:11ZThe P aracoccus denitrificans NarK-like nitrate and nitrite transporters—probing nitrate uptake and nitrate/nitrite exchange mechanismsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:edd7e44f-6d5a-47c6-91bd-55325377efe4Symplectic Elements at OxfordWiley2016Ferguson, SGoddard, ABali, SMavridou, DLuque-Almagro, VGates, ARoldan, MNewstead, SRichardson, DNitrate and nitrite transport across biological membranes is often facilitated by protein transporters that are members of the major facilitator superfamily. Paracoccus denitrificans contains an unusual arrangement whereby two of these transporters, NarK1 and NarK2, are fused into a single protein, NarK, which delivers nitrate to the respiratory nitrate reductase and transfers the product, nitrite, to the periplasm. Our complementation studies, using a mutant lacking the nitrate/proton symporter NasA from the assimilatory nitrate reductase pathway, support that NarK1 functions as a nitrate/proton symporter while NarK2 is a nitrate/nitrite antiporter. Through the same experimental system, we find that Escherichia coli NarK and NarU can complement deletions in both narKand nasAin P. denitrificans, suggesting that, while these proteins are most likely nitrate/nitrite antiporters, they can also act in the net uptake of nitrate. Finally, we argue that primary sequence analysis and structural modelling do not readily explain why NasA, NarK1 and NarK2, as well as othertransporters from this protein family, have such different functions, ranging fromnet nitrate uptake to nitrate/nitrite exchange. |
| spellingShingle | Ferguson, S Goddard, A Bali, S Mavridou, D Luque-Almagro, V Gates, A Roldan, M Newstead, S Richardson, D The P aracoccus denitrificans NarK-like nitrate and nitrite transporters—probing nitrate uptake and nitrate/nitrite exchange mechanisms |
| title | The P aracoccus denitrificans NarK-like nitrate and nitrite transporters—probing nitrate uptake and nitrate/nitrite exchange mechanisms |
| title_full | The P aracoccus denitrificans NarK-like nitrate and nitrite transporters—probing nitrate uptake and nitrate/nitrite exchange mechanisms |
| title_fullStr | The P aracoccus denitrificans NarK-like nitrate and nitrite transporters—probing nitrate uptake and nitrate/nitrite exchange mechanisms |
| title_full_unstemmed | The P aracoccus denitrificans NarK-like nitrate and nitrite transporters—probing nitrate uptake and nitrate/nitrite exchange mechanisms |
| title_short | The P aracoccus denitrificans NarK-like nitrate and nitrite transporters—probing nitrate uptake and nitrate/nitrite exchange mechanisms |
| title_sort | p aracoccus denitrificans nark like nitrate and nitrite transporters probing nitrate uptake and nitrate nitrite exchange mechanisms |
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