The DNA transporter ComEC has metal-dependent nuclease activity that is important for natural transformation
In the process of natural transformation bacteria import extracellular DNA molecules for integration into their genome. One strand of the incoming DNA molecule is degraded, whereas the remaining strand is transported across the cytoplasmic membrane. The DNA transport channel is provided by the prote...
| Hoofdauteurs: | , , |
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| Formaat: | Journal article |
| Taal: | English |
| Gepubliceerd in: |
Wiley
2021
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| _version_ | 1826303722627530752 |
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| author | Silale, A Lea, S Berks, B |
| author_facet | Silale, A Lea, S Berks, B |
| author_sort | Silale, A |
| collection | OXFORD |
| description | In the process of natural transformation bacteria import extracellular DNA molecules for integration into their genome. One strand of the incoming DNA molecule is degraded, whereas the remaining strand is transported across the cytoplasmic membrane. The DNA transport channel is provided by the protein ComEC. Many ComEC proteins have an extracellular C-terminal domain (CTD) with homology to the metallo-β-lactamase fold. Here we show that this CTD binds Mn2+ ions and exhibits Mn2+-dependent phosphodiesterase and nuclease activities. Inactivation of the enzymatic activity of the CTD severely inhibits natural transformation in Bacillus subtilis. These data suggest that the ComEC CTD is a nuclease responsible for degrading the nontransforming DNA strand during natural transformation and that this process is important for efficient DNA import. |
| first_indexed | 2024-03-07T06:07:02Z |
| format | Journal article |
| id | oxford-uuid:ee32ab3a-11b7-4255-8c90-3ab5f0645461 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:07:02Z |
| publishDate | 2021 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:ee32ab3a-11b7-4255-8c90-3ab5f06454612022-03-27T11:30:56ZThe DNA transporter ComEC has metal-dependent nuclease activity that is important for natural transformationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ee32ab3a-11b7-4255-8c90-3ab5f0645461EnglishSymplectic ElementsWiley2021Silale, ALea, SBerks, BIn the process of natural transformation bacteria import extracellular DNA molecules for integration into their genome. One strand of the incoming DNA molecule is degraded, whereas the remaining strand is transported across the cytoplasmic membrane. The DNA transport channel is provided by the protein ComEC. Many ComEC proteins have an extracellular C-terminal domain (CTD) with homology to the metallo-β-lactamase fold. Here we show that this CTD binds Mn2+ ions and exhibits Mn2+-dependent phosphodiesterase and nuclease activities. Inactivation of the enzymatic activity of the CTD severely inhibits natural transformation in Bacillus subtilis. These data suggest that the ComEC CTD is a nuclease responsible for degrading the nontransforming DNA strand during natural transformation and that this process is important for efficient DNA import. |
| spellingShingle | Silale, A Lea, S Berks, B The DNA transporter ComEC has metal-dependent nuclease activity that is important for natural transformation |
| title | The DNA transporter ComEC has metal-dependent nuclease activity that is important for natural transformation |
| title_full | The DNA transporter ComEC has metal-dependent nuclease activity that is important for natural transformation |
| title_fullStr | The DNA transporter ComEC has metal-dependent nuclease activity that is important for natural transformation |
| title_full_unstemmed | The DNA transporter ComEC has metal-dependent nuclease activity that is important for natural transformation |
| title_short | The DNA transporter ComEC has metal-dependent nuclease activity that is important for natural transformation |
| title_sort | dna transporter comec has metal dependent nuclease activity that is important for natural transformation |
| work_keys_str_mv | AT silalea thednatransportercomechasmetaldependentnucleaseactivitythatisimportantfornaturaltransformation AT leas thednatransportercomechasmetaldependentnucleaseactivitythatisimportantfornaturaltransformation AT berksb thednatransportercomechasmetaldependentnucleaseactivitythatisimportantfornaturaltransformation AT silalea dnatransportercomechasmetaldependentnucleaseactivitythatisimportantfornaturaltransformation AT leas dnatransportercomechasmetaldependentnucleaseactivitythatisimportantfornaturaltransformation AT berksb dnatransportercomechasmetaldependentnucleaseactivitythatisimportantfornaturaltransformation |