Crystallization and crystal packing of recombinant 3 (or 17) beta-hydroxysteroid dehydrogenase from Comamonas testosteroni ATTC 11996.
The enzyme 3 (or 17) beta-hydroxysteroid dehydrogenase from Comamonas testosteroni was crystallized. Crystals, of up to 0.6 mm in their longest dimension and suitable for a crystallographic analysis have been obtained by the vapour diffusion method. They belong to the orthorhombic lattice type and d...
| Hauptverfasser: | , , , , , |
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| Format: | Journal article |
| Sprache: | English |
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Blackwell Publishing Ltd
1996
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| _version_ | 1826303876089774080 |
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| author | Benach, J Knapp, S Oppermann, U Hägglund, O Jörnvall, H Ladenstein, R |
| author_facet | Benach, J Knapp, S Oppermann, U Hägglund, O Jörnvall, H Ladenstein, R |
| author_sort | Benach, J |
| collection | OXFORD |
| description | The enzyme 3 (or 17) beta-hydroxysteroid dehydrogenase from Comamonas testosteroni was crystallized. Crystals, of up to 0.6 mm in their longest dimension and suitable for a crystallographic analysis have been obtained by the vapour diffusion method. They belong to the orthorhombic lattice type and diffract to a maximum resolution of 0.23 nm. A final data set obtained by merging data from three crystals resulted in a completeness of 90% with an Rmerge of 6%. A molecular replacement search carried out by using 3 alpha (or 20 beta)-hydroxysteroid dehydrogenase from Streptomyces hydrogenans as a search model allowed us to assign I222 as the correct space group and to propose a model for the crystal packing, with one monomer per asymmetric unit. Thus, the whole unit cell contains two tetramers. The R-factor after rigid body refinement is 48.1%. |
| first_indexed | 2024-03-07T06:09:21Z |
| format | Journal article |
| id | oxford-uuid:eeefcc06-8473-4a80-a33c-e15fda3b30d2 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:09:21Z |
| publishDate | 1996 |
| publisher | Blackwell Publishing Ltd |
| record_format | dspace |
| spelling | oxford-uuid:eeefcc06-8473-4a80-a33c-e15fda3b30d22022-03-27T11:36:34ZCrystallization and crystal packing of recombinant 3 (or 17) beta-hydroxysteroid dehydrogenase from Comamonas testosteroni ATTC 11996.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:eeefcc06-8473-4a80-a33c-e15fda3b30d2EnglishSymplectic Elements at OxfordBlackwell Publishing Ltd1996Benach, JKnapp, SOppermann, UHägglund, OJörnvall, HLadenstein, RThe enzyme 3 (or 17) beta-hydroxysteroid dehydrogenase from Comamonas testosteroni was crystallized. Crystals, of up to 0.6 mm in their longest dimension and suitable for a crystallographic analysis have been obtained by the vapour diffusion method. They belong to the orthorhombic lattice type and diffract to a maximum resolution of 0.23 nm. A final data set obtained by merging data from three crystals resulted in a completeness of 90% with an Rmerge of 6%. A molecular replacement search carried out by using 3 alpha (or 20 beta)-hydroxysteroid dehydrogenase from Streptomyces hydrogenans as a search model allowed us to assign I222 as the correct space group and to propose a model for the crystal packing, with one monomer per asymmetric unit. Thus, the whole unit cell contains two tetramers. The R-factor after rigid body refinement is 48.1%. |
| spellingShingle | Benach, J Knapp, S Oppermann, U Hägglund, O Jörnvall, H Ladenstein, R Crystallization and crystal packing of recombinant 3 (or 17) beta-hydroxysteroid dehydrogenase from Comamonas testosteroni ATTC 11996. |
| title | Crystallization and crystal packing of recombinant 3 (or 17) beta-hydroxysteroid dehydrogenase from Comamonas testosteroni ATTC 11996. |
| title_full | Crystallization and crystal packing of recombinant 3 (or 17) beta-hydroxysteroid dehydrogenase from Comamonas testosteroni ATTC 11996. |
| title_fullStr | Crystallization and crystal packing of recombinant 3 (or 17) beta-hydroxysteroid dehydrogenase from Comamonas testosteroni ATTC 11996. |
| title_full_unstemmed | Crystallization and crystal packing of recombinant 3 (or 17) beta-hydroxysteroid dehydrogenase from Comamonas testosteroni ATTC 11996. |
| title_short | Crystallization and crystal packing of recombinant 3 (or 17) beta-hydroxysteroid dehydrogenase from Comamonas testosteroni ATTC 11996. |
| title_sort | crystallization and crystal packing of recombinant 3 or 17 beta hydroxysteroid dehydrogenase from comamonas testosteroni attc 11996 |
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