Scanning electron microscopy of cells and tissues under fully hydrated conditions.
A capability for scanning electron microscopy of wet biological specimens is presented. A membrane that is transparent to electrons protects the fully hydrated sample from the vacuum. The result is a hybrid technique combining the ease of use and ability to see into cells of optical microscopy with...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2004
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| _version_ | 1826303892984430592 |
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| author | Thiberge, S Nechushtan, A Sprinzak, D Gileadi, O Behar, V Zik, O Chowers, Y Michaeli, S Schlessinger, J Moses, E |
| author_facet | Thiberge, S Nechushtan, A Sprinzak, D Gileadi, O Behar, V Zik, O Chowers, Y Michaeli, S Schlessinger, J Moses, E |
| author_sort | Thiberge, S |
| collection | OXFORD |
| description | A capability for scanning electron microscopy of wet biological specimens is presented. A membrane that is transparent to electrons protects the fully hydrated sample from the vacuum. The result is a hybrid technique combining the ease of use and ability to see into cells of optical microscopy with the higher resolution of electron microscopy. The resolution of low-contrast materials is approximately 100 nm, whereas in high-contrast materials the resolution can reach 10 nm. Standard immunogold techniques and heavy-metal stains can be applied and viewed in the fluid to improve the contrast. Images present a striking combination of whole-cell morphology with a wealth of internal details. A possibility for direct inspection of tissue slices transpires, imaging only the external layer of cells. Simultaneous imaging with photons excited by the electrons incorporates data on material distribution, indicating a potential for multilabeling and specific scintillating markers. |
| first_indexed | 2024-03-07T06:09:36Z |
| format | Journal article |
| id | oxford-uuid:ef04b566-f3a9-4832-ba64-43c3bebe795c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T06:09:36Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:ef04b566-f3a9-4832-ba64-43c3bebe795c2022-03-27T11:37:13ZScanning electron microscopy of cells and tissues under fully hydrated conditions.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ef04b566-f3a9-4832-ba64-43c3bebe795cEnglishSymplectic Elements at Oxford2004Thiberge, SNechushtan, ASprinzak, DGileadi, OBehar, VZik, OChowers, YMichaeli, SSchlessinger, JMoses, EA capability for scanning electron microscopy of wet biological specimens is presented. A membrane that is transparent to electrons protects the fully hydrated sample from the vacuum. The result is a hybrid technique combining the ease of use and ability to see into cells of optical microscopy with the higher resolution of electron microscopy. The resolution of low-contrast materials is approximately 100 nm, whereas in high-contrast materials the resolution can reach 10 nm. Standard immunogold techniques and heavy-metal stains can be applied and viewed in the fluid to improve the contrast. Images present a striking combination of whole-cell morphology with a wealth of internal details. A possibility for direct inspection of tissue slices transpires, imaging only the external layer of cells. Simultaneous imaging with photons excited by the electrons incorporates data on material distribution, indicating a potential for multilabeling and specific scintillating markers. |
| spellingShingle | Thiberge, S Nechushtan, A Sprinzak, D Gileadi, O Behar, V Zik, O Chowers, Y Michaeli, S Schlessinger, J Moses, E Scanning electron microscopy of cells and tissues under fully hydrated conditions. |
| title | Scanning electron microscopy of cells and tissues under fully hydrated conditions. |
| title_full | Scanning electron microscopy of cells and tissues under fully hydrated conditions. |
| title_fullStr | Scanning electron microscopy of cells and tissues under fully hydrated conditions. |
| title_full_unstemmed | Scanning electron microscopy of cells and tissues under fully hydrated conditions. |
| title_short | Scanning electron microscopy of cells and tissues under fully hydrated conditions. |
| title_sort | scanning electron microscopy of cells and tissues under fully hydrated conditions |
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