Transposable elements contribute to the establishment of the glycine shuttle in Brassicaceae species
<ul> <li>C<sub>3</sub>-C<sub>4</sub> intermediate photosynthesis has evolved at least five times convergently in the Brassicaceae, despite this family lacking <em>bona fide</em> C<sub>4</sub> species. The...
| Päätekijät: | , , , , , , , , , , |
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| Aineistotyyppi: | Journal article |
| Kieli: | English |
| Julkaistu: |
Wiley
2024
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| _version_ | 1826312917220327424 |
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| author | Triesch, S Denton, AK Bouvier, JW Buchmann, JP Reichel-Deland, V Guerreiro, RNFM Busch, N Schlüter, U Stich, B Kelly, S Weber, APM |
| author_facet | Triesch, S Denton, AK Bouvier, JW Buchmann, JP Reichel-Deland, V Guerreiro, RNFM Busch, N Schlüter, U Stich, B Kelly, S Weber, APM |
| author_sort | Triesch, S |
| collection | OXFORD |
| description | <ul>
<li>C<sub>3</sub>-C<sub>4</sub> intermediate photosynthesis has evolved at least five times convergently in the Brassicaceae, despite this family lacking <em>bona fide</em> C<sub>4</sub> species. The establishment of this carbon concentrating mechanism is known to require a complex suite of ultrastructural modifications, as well as changes in spatial expression patterns, which are both thought to be underpinned by a reconfiguration of existing gene-regulatory networks. However, to date, the mechanisms which underpin the reconfiguration of these gene networks are largely unknown.</li>
<li>In this study, we used a pan-genomic association approach to identify genomic features that could confer differential gene expression towards the C<sub>3</sub>-C<sub>4</sub> intermediate state by analysing eight C<sub>3</sub> species and seven C<sub>3</sub>-C<sub>4</sub> species from five independent origins in the Brassicaceae.</li>
<li>We found a strong correlation between transposable element (TE) insertions in <em>cis</em>-regulatory regions and C<sub>3</sub>-C<sub>4</sub> intermediacy. Specifically, our study revealed 113 gene models in which the presence of a TE within a gene correlates with C<sub>3</sub>-C<sub>4</sub> intermediate photosynthesis. In this set, genes involved in the photorespiratory glycine shuttle are enriched, including the glycine decarboxylase P-protein whose expression domain undergoes a spatial shift during the transition to C<sub>3</sub>-C<sub>4</sub> photosynthesis. When further interrogating this gene, we discovered independent TE insertions in its upstream region which we conclude to be responsible for causing the spatial shift in <em>GLDP1</em> gene expression.</li>
<li>Our findings hint at a pivotal role of TEs in the evolution of C<sub>3</sub>-C<sub>4</sub> intermediacy, especially in mediating differential spatial gene expression.</li>
</ul> |
| first_indexed | 2024-03-07T08:22:25Z |
| format | Journal article |
| id | oxford-uuid:ef9230ec-d9da-41ed-b0da-306db30aecac |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-09-25T04:02:45Z |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:ef9230ec-d9da-41ed-b0da-306db30aecac2024-05-01T09:52:30ZTransposable elements contribute to the establishment of the glycine shuttle in Brassicaceae speciesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ef9230ec-d9da-41ed-b0da-306db30aecacEnglishSymplectic ElementsWiley2024Triesch, SDenton, AKBouvier, JWBuchmann, JPReichel-Deland, VGuerreiro, RNFMBusch, NSchlüter, UStich, BKelly, SWeber, APM<ul> <li>C<sub>3</sub>-C<sub>4</sub> intermediate photosynthesis has evolved at least five times convergently in the Brassicaceae, despite this family lacking <em>bona fide</em> C<sub>4</sub> species. The establishment of this carbon concentrating mechanism is known to require a complex suite of ultrastructural modifications, as well as changes in spatial expression patterns, which are both thought to be underpinned by a reconfiguration of existing gene-regulatory networks. However, to date, the mechanisms which underpin the reconfiguration of these gene networks are largely unknown.</li> <li>In this study, we used a pan-genomic association approach to identify genomic features that could confer differential gene expression towards the C<sub>3</sub>-C<sub>4</sub> intermediate state by analysing eight C<sub>3</sub> species and seven C<sub>3</sub>-C<sub>4</sub> species from five independent origins in the Brassicaceae.</li> <li>We found a strong correlation between transposable element (TE) insertions in <em>cis</em>-regulatory regions and C<sub>3</sub>-C<sub>4</sub> intermediacy. Specifically, our study revealed 113 gene models in which the presence of a TE within a gene correlates with C<sub>3</sub>-C<sub>4</sub> intermediate photosynthesis. In this set, genes involved in the photorespiratory glycine shuttle are enriched, including the glycine decarboxylase P-protein whose expression domain undergoes a spatial shift during the transition to C<sub>3</sub>-C<sub>4</sub> photosynthesis. When further interrogating this gene, we discovered independent TE insertions in its upstream region which we conclude to be responsible for causing the spatial shift in <em>GLDP1</em> gene expression.</li> <li>Our findings hint at a pivotal role of TEs in the evolution of C<sub>3</sub>-C<sub>4</sub> intermediacy, especially in mediating differential spatial gene expression.</li> </ul> |
| spellingShingle | Triesch, S Denton, AK Bouvier, JW Buchmann, JP Reichel-Deland, V Guerreiro, RNFM Busch, N Schlüter, U Stich, B Kelly, S Weber, APM Transposable elements contribute to the establishment of the glycine shuttle in Brassicaceae species |
| title | Transposable elements contribute to the establishment of the glycine shuttle in Brassicaceae species |
| title_full | Transposable elements contribute to the establishment of the glycine shuttle in Brassicaceae species |
| title_fullStr | Transposable elements contribute to the establishment of the glycine shuttle in Brassicaceae species |
| title_full_unstemmed | Transposable elements contribute to the establishment of the glycine shuttle in Brassicaceae species |
| title_short | Transposable elements contribute to the establishment of the glycine shuttle in Brassicaceae species |
| title_sort | transposable elements contribute to the establishment of the glycine shuttle in brassicaceae species |
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