Fingerprinting of falsified artemisinin combination therapies via direct analysis in real time coupled to a compact single quadrupole mass spectrometer
Falsified anti-malarial treatments continue to constitute a major health crisis, especially in malarious Africa. Even after detection of poor quality pharmaceuticals, it is critical that they be fully analyzed to determine their components, in order to assess their health effects and ultimately allo...
| Main Authors: | , , , , |
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| Format: | Journal article |
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Royal Society of Chemistry
2016
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| _version_ | 1797102926076837888 |
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| author | Bernier, M Li, F Musselman, B Newton, P Fernández, F |
| author_facet | Bernier, M Li, F Musselman, B Newton, P Fernández, F |
| author_sort | Bernier, M |
| collection | OXFORD |
| description | Falsified anti-malarial treatments continue to constitute a major health crisis, especially in malarious Africa. Even after detection of poor quality pharmaceuticals, it is critical that they be fully analyzed to determine their components, in order to assess their health effects and ultimately allow forensic tracing of their sources of production and distribution. Timely assessment requires robust and complete field-testing, or at the very least timely analysis after seizure or purchase. Ideally, low-cost and simple analytical equipment such as portable mass spectrometry (MS) is the best approach for achieving this quick and informative analysis. To date, Direct Analysis in Real Time (DART) MS has been successfully implemented to rapidly analyze falsified artemisinin-based combination therapies (ACTs) in laboratory settings, but this approach typically translates into high-cost and the need for high-resolution instrumentation. Here, we examine the use of DART ionization coupled with a portable low-resolution single-quadrupole instrument, and compare its success in fingerprinting anti-malarial tablets with higher resolution instrumentation. Using single quadrupole DART-MS, the same sample components were detected as with the high-resolution instrument, while needing significantly less consumables and power, and the additional advantages of increased portability and ease of use. Using Principal Component Analysis (PCA) of DART data, specific classes of falsified ACTs were identified, providing a more straightforward method for sourcing counterfeits and assessing their similarities. |
| first_indexed | 2024-03-07T06:12:46Z |
| format | Journal article |
| id | oxford-uuid:f017b17e-7dae-45cd-ac09-0410a958fbf0 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T06:12:46Z |
| publishDate | 2016 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | oxford-uuid:f017b17e-7dae-45cd-ac09-0410a958fbf02022-03-27T11:45:08ZFingerprinting of falsified artemisinin combination therapies via direct analysis in real time coupled to a compact single quadrupole mass spectrometerJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:f017b17e-7dae-45cd-ac09-0410a958fbf0Symplectic Elements at OxfordRoyal Society of Chemistry2016Bernier, MLi, FMusselman, BNewton, PFernández, FFalsified anti-malarial treatments continue to constitute a major health crisis, especially in malarious Africa. Even after detection of poor quality pharmaceuticals, it is critical that they be fully analyzed to determine their components, in order to assess their health effects and ultimately allow forensic tracing of their sources of production and distribution. Timely assessment requires robust and complete field-testing, or at the very least timely analysis after seizure or purchase. Ideally, low-cost and simple analytical equipment such as portable mass spectrometry (MS) is the best approach for achieving this quick and informative analysis. To date, Direct Analysis in Real Time (DART) MS has been successfully implemented to rapidly analyze falsified artemisinin-based combination therapies (ACTs) in laboratory settings, but this approach typically translates into high-cost and the need for high-resolution instrumentation. Here, we examine the use of DART ionization coupled with a portable low-resolution single-quadrupole instrument, and compare its success in fingerprinting anti-malarial tablets with higher resolution instrumentation. Using single quadrupole DART-MS, the same sample components were detected as with the high-resolution instrument, while needing significantly less consumables and power, and the additional advantages of increased portability and ease of use. Using Principal Component Analysis (PCA) of DART data, specific classes of falsified ACTs were identified, providing a more straightforward method for sourcing counterfeits and assessing their similarities. |
| spellingShingle | Bernier, M Li, F Musselman, B Newton, P Fernández, F Fingerprinting of falsified artemisinin combination therapies via direct analysis in real time coupled to a compact single quadrupole mass spectrometer |
| title | Fingerprinting of falsified artemisinin combination therapies via direct analysis in real time coupled to a compact single quadrupole mass spectrometer |
| title_full | Fingerprinting of falsified artemisinin combination therapies via direct analysis in real time coupled to a compact single quadrupole mass spectrometer |
| title_fullStr | Fingerprinting of falsified artemisinin combination therapies via direct analysis in real time coupled to a compact single quadrupole mass spectrometer |
| title_full_unstemmed | Fingerprinting of falsified artemisinin combination therapies via direct analysis in real time coupled to a compact single quadrupole mass spectrometer |
| title_short | Fingerprinting of falsified artemisinin combination therapies via direct analysis in real time coupled to a compact single quadrupole mass spectrometer |
| title_sort | fingerprinting of falsified artemisinin combination therapies via direct analysis in real time coupled to a compact single quadrupole mass spectrometer |
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